Effects of metformin, rapamycin, and resveratrol on cellular metabolism of canine primary fibroblast cells isolated from large and small breeds as they age
We found that rapa and res increased rates of non-glycolytic acidification in small and large breed puppies and basal oxygen consumption rates (OCR) in small and large breed puppies. Rapa increased proton leak and non-mitochondrial respiration in small and large breed puppies. Maximal respiration was significantly altered with rapa treatment but in opposing ways: large breed puppies showed a significant increase in maximal respiration when treated with rapa, and small old dogs demonstrated a significant decrease in maximal respiration when treated with rapa. In opposition to rapa treatments, met significantly decreased basal OCR levels in cells from small and large breed puppies. Our data suggest that rapa treatments may be metabolically beneficial to dogs when started early in life and more beneficial in larger breeds.
From the whole article as much I understood, I thought this wasn’t good for small old dogs. Then I asked in another forum and someone did a summary and said It looks like Rapamycin may be detrimental to older smaller dogs.
Large Breeds: Benefit significantly from early-life treatment due to improvements in mitochondrial function.
Small Breeds: While puppies benefit from enhanced metabolism, older dogs may experience reduced mitochondrial capacity with treatment.
But I also read real life stories that even smaller/older dogs benefited from Rapa. I don’t want to miss the potential good for my dog but I also don’t want to give him problems.
Rapamycin does a lot of things at different doses. You can focus on one small issue at a specific dose in a specific situation but I think you have to look at the overall results in every species and model organism it’s been tested in. In virtually every situation the medium and maximum lifespans increase over the control groups.
Of course not every one of the treated group lives longer. In all of this we are taking a probabilistic approach. The good news is that we can monitor our dogs and ourselves (who are taking the rapamycin) can pause or adjust if the results are not what we want
I was just saying that I think my 3 elderly cats are much perkier since starting on rapa
Well, one cat just attacked another one, ripping out hair and causing a lot of bleeding. This has never happened in 17 years. It then dawned on me you mentioned your dog become more aggressive. Now I wonder if it’s related. Sigh.
Has anyone heard of this happening in the dog study?
In Kaeberlein’s study only non-reactive dogs are for candidates for the trial.
I am using rapamycin for my companion animal in coordination with a veterinarian who is, himself, a researcher and has experience with rapamycin. Again, if you do not have access to this type of veterinary care locally, you might want to consider PetSpan. You can see Dan’s comments above.
Kelsey, I really appreciate your concern, that is very kind of you. Thank you for making sure my cats have good care. Because you’ve shown your concern twice, I’ll share that I am fortunate to have one of the top IM vets in NorCal, and she is familiar with rapamycin.
I am simply on here just to share information and ask questions, similar to what many forum members do, even those who are fortunate enough to have excellent doctors who give great advice on rapamycin.
It might wind up being helpful for those who see something unexpected, like the incident today from a very mild mannered cat, to share it here incase we eventually see a trend. That is why I was curious if anyone knew if it happened in the dog study. If anyone has heard, do let me know because one incident at my house after 17 years does not show any sort of connection. It’s just a wonder at this point. Thanks
So article got me thinking… dogs can sense disease like cancers.
The several dogs that I helped get on rapamycin have truly shown reversal in health and activity. They are reinvested in activities… sniffing… their best sense and acting years younger. Dry scabbed noses return to healthy wetness. Cancer lumps disapate. They seem truly thankful to their owners.
If they can smell disease in humans… I am guessing they smell their own return to health.
Dog Owners using Rapamycin - please contact me (via Direct message - click on my avatar and then “message” me) if this is potentially of interest. My dog is too young. I think the best person would be a person who has been giving rapamycin to an older dog for at least a year and has some positive results that they can report.
Ideally the person would be in Seattle area, but if not, then the NY area, or other major city (I suspect) - but they are willing to travel, so just include your location in your email to me and I’ll let them figure out the best fit for their needs.
My name is Koji Hayasaki, and I am a TV documentary coordinator based in
New York. I am currently working with NHK, Japan’s public television
broadcaster, on a special documentary focused on anti-aging.
I am trying to follow up my initial inquiry email on May 7th.
This program will explore the latest advancements in anti-aging research
and technology, featuring the work of scientists and entrepreneurs
leading the field. One segment will focus on the science and effects of
rapamycin, including a feature on the Dog Aging Project at Washington
University. As part of this, we will be interviewing Dr. Matt Kaeberlein.
To help our audience better understand the potential health benefits of
rapamycin for dogs, we would like to feature a real-life example of a
dog whose health has improved after taking the drug. I came across
Rapamycin News and a thread discussing dogs’ use of rapamycin:
I’m reaching out to ask whether the NHK team could film you and your dog
for this segment, and if you’d be willing to share your experience. We
plan to film in the U.S. between late August and September, and NHK
would be able to offer compensation for your time.
Thank you very much for considering this opportunity. I look forward to
hearing from you.
I bought rapamycin from a lab, 99.5% purity for animal use.
I’m currently giving 0.75 or 1mg rapa to my 10kg 7.5-years-old dog biweekly.
Have used for 4 month.
Haven’t seen any difference yet, but he does look more energetic.
Are there any test that can do to dog to check if there is any antiaging effect?
Unfortunately there are no tests for anti-aging effects yet (not really in humans either)… so we’re all in the dark.
How are you giving the rapamycin powder to the dog (is it in an enteric encapsulated capsule?). I ask because rapamycin as a powder is not very bioavailable and almost all of it gets destroyed in the gut if its not protected. (standard capsules don’t work). See these threads:
For now I can only buy rapa in powder form, so I could only give it to my dog with food.
Bioavailability is a big problem, but I do find Sirolimus Oral Solution which is in liquid form, clinically it is effective too.
I don’t know if it is OK to increase dose to offset the loss in stomach.
Yes - it would definitely be advised to increase dose based on stomach loss… at least triple the dose because I think the bioavailability is only 1/3 or lower of the protected rapamycin.
Thank you Beth
I tried enteric capsules or other similar container for my dog once, but my dog is not a really big one so he would chew and probably break the capsule before swallow it
So difficult to make him eat meds.
What’s more in my place, sirolimus in tablets form is much much more expensive than powder form
That all makes sense. I guess this means you are not in the US, if you are, let me know and I’ll tell you how to get it cheaper.
Have you ever tried pilling him? (With your homemade capsules)
I have two cats I can pill. It would be too traumatic for my third cat, so unless something was life saving, it wouldn’t do that to him.
I’m happy to do a FaceTime and teach you how to pill him if you think that would help (you buy a little cheap tool and approach from behind and go in the side of his mouth, so he never sees it coming). I just taught a fried how to pill their cat last week and their jaw was on the floor over how quick and easy it was.
