got 4th report from 48 hours after 4.3 and down to 1.7, implied half-life of 36 hours from that starting level. Makes sense and consistent with chart posted above (or other thread?) that half-life longer from lower starting value.
I’m a little unclear what the ideal values for Tmax and trough level before next dose I should be aiming for. I’ll have to dig around in this thread and here to investigate. I prob saw some hints that didn’t sink in before I started my testing.
Oh, I forgot to mention, I also take CBD and Theracumin(Curcumin), and iirc the former should inhibit Rapa metabolization and the latter seems to increase it and greatly lower AUC: Turmeric Linked to Liver Damage, Decrease in Rapamycin Bioavailability. (Though confusingly I’ve seen it both ways, that Curcumin both inhibits and activates the relevant CYP pathway. I’ll post in the thread about that…)
[side note on CBD. I took it to help sleep, but it cured my Morton’s Neuroma (nerve pain in foot) immediately! I’d had annoying pain in the morning literally every single day for over a year, and a Dr rec’d CDB for sleep, so I gave it a try, and the next morning after being awake for a while I realized that my foot hadn’t hurt at all. So I’ve been taking it every since and the pain hasn’t returned. And it can’t be placebo, because I took it for sleep and it never occurred to me it would help with foot pain…]
Yes - you should be a little unclear… nobody really knows for sure. On the Tmax level, in the NIA ITP mouse studies, generally the higher the dose, the better the lifespan increase. In one study ( of the 45+ studies of rapamycin in mice) they did finally find an upper dose of rapamycin that no longer increased lifespan in those female mice. It was an extremely high dose though.
I should note that in mice there is a significant dose / response difference between male mice and female mice. This difference is not seen in humans.
Most of people currently using rapamycin seem to be (in mouse equivalent dosing) in the low levels tested (note: mice were dosed rapamycin in their food, daily, so that is another difference - vs. us using rapamycin dosed once weekly or so).
Here are the National Institutes on Aging Results from their rapamycin studies (these are the best rapamycin studies):
Sirolimus Dose
mg/kg/day Dose
Blood/Sirolimus Level
Male Median LS Increase
Female Median LS Increase
4.7ppm
∼2.24
3 to 4 ng/mL
3%
16%
14ppm
~6.67
9-16 ng/mL
13%
21%
42ppm
~20
23-80 ng/mL
23%
26%
Here are results from all the higher dose studies I could find:
Here’s my result. This is about 90 minutes after taking 2 1mg Biocon pills together with 2 1mg Zydus pills with half a small red grapefruit, 2Tbs of EVOO, washed down with a small amount of pasteurized red grapefruit juice. The test result also puts to rest the notion that the Indian product is fake or ineffective.
Sirolimus (Rapamune), Blood
Test Current Result
Sirolimus, Blood 31.8 ng/mL Reference interval 3.0-20.0
Ok, finally I got some proper absorption leading to solid peak blood level.
I think the relevant change was stopping my Theracumin/Curcumin for 10+ days. I also slightly altered two other things: this time I ate a grapefruit the night before AND and hour before, vs just 1 gf an hour before last time, and I took a much bigger swig or two of olive oil with the dose, as opposed to a smaller but still solid swing last time:
(I also upped the dose from 10 to 12, but that change is easy to account for.)
Oh, also for good measure I waited 3 hours to test rather than just over 1 hour the previous time, in case I was just a slow absorber. Figure I’d get closer to peak because making sure it was fully absorbed would overwhelm the little bit of metabolism I’d do in the 2 hours.
This time I hit 43.6, vs 15.6 last time. (Normalizing 12mg to 10 mg dose: 36.3 v 15.6):
Still seems like I absorb less than @Agetron, but I’ll take this result and will make this my new protocol, though I may play with the dose more. And at least I know the pills I’m taking are good.
Doing another test today (5 days later) to get another read-out on my half-life now that I eliminated Curcumin…stay tuned.
ps no noticeable side effects at this blood level for me…
Kind of shocking to get that warning… 8mg and a glass of GFJ - I hit 56.9 ng/mL – whoops - a 7x’s increase with the GFJ. And, had diarrhea for a few days. Just goes to show how we all aborb rapamycin differently.
Labcorp test July 18, 2022 56.9 ng/mL
Having Labcorp results… like me - you are better able to control the dose with GFJ. I will get my blood tested again in a month (April 2023) and do 2mg rapamycin and one Red Grapefuit squeezed for juice. If I am consistent - I should get a 12 ng/mL result which is what I am shooting for as my current dose.
I should have my TruDiagnositc results soon and can see if the reduced rapamycin from 36 ng/mL the 7 months to 12 ng/mL for the past 3 months has been better for my biological age.
After computing my body surface area (BSA) of 1.73 sqm as described in the Siroboon thread, I get 5.8mg/sqm. Relative to this chart, it looks like my peak should be somewhat above 50ng/mL.
I have one last lab test scheduled for this Friday, 3/10/23, this time using Siroboon.
PS Always google for a Marek discount coupon. You should find something for 10% off.
I have to believe that there is some lab technician in these companies wondering what the hell is going on with all these extremely high blood measures of sirolimus they have suddenly started seeing in the past year…
that is difficult to see that must be checked to process.
