Zydus passes Labcorp test

#41

Hahaha, okay… your Zydus shows the molecule rapamycin is in your blood. Congrats.

Unclear how you are getting your numbers.
Typically a person goes for a full week after last dose. Your trough… typically a week later you would be under 1ng/mL. You say you are at 10.6ng/ml at trough?

Did you mean you had 10.6ng/ml 3 hours after your dose? That would make more sense.

Take your Zydus pills (4mg or 6mg)… then get blood tested no earlier than 2 hours or later than 3 hours is good.

You should be getting about 3 ng/mL for every 1 mg of Zydus 2-3 hours after your dose. So at 5mg you should be at 15 ng/mL.

As to SiroBoon… sounds lke it is crap… everyone on here tests and no rapamycin in it.

Your earlier post:
“As of now having been taking rapa for many months only to discover bogus meds. Biodin and Zydus.”

So… now you believe your Zydus is good.

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#42

There is lots of individual variation in this… and we have no idea what @CoachHarvey takes in the way of drugs, supplements or foods in the hours prior to dosing with sirolimus… Rapamycin Interactions with Other Food, Drinks, Supplements and Drugs

Key Pharmacokinetic Insights

  • Rapid Absorption: Under fasting conditions, sirolimus is absorbed quickly, reaching peak blood levels (Tmax​) within approximately 1 hour.
  • Dose Proportionality: In the therapeutic range, Cmax​ increases roughly linearly with the dose. A 5 mg dose typically results in a peak concentration about 2.5 times higher than a 2 mg dose.
  • Food Effect: Consuming a high-fat meal significantly alters these parameters. It can delay Tmax​ (up to 2.5–3.0 hours), decrease Cmax​ by ~34%, and increase total exposure (AUC) by ~35%. The graph above models the fasting state.
  • Elimination: After the peak, levels drop relatively slowly due to the long half-life (~62 hours in healthy males), meaning a single dose remains detectable in the blood for several days.

Scholarly Note on Variability: While these curves represent the “typical” male, actual blood levels are heavily influenced by the activity of the CYP3A4 and CYP3A5 enzymes and the P-glycoprotein transporter. Individuals with high metabolic activity (“rapid metabolizers”) may see lower Cmax​ values, while those with inhibited pathways (or taking CYP3A inhibitors like grapefruit juice/ketoconazole) may experience significantly higher spikes.

Sources: Clinical Pharmacology Biopharmaceutics Review (Rapamune), PubMed (PMID: 11034258, 16418694).

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#43

Do you a gree that weekly dose that leave near 0ng/ml at trough is better than twice weekly 1/2 per dose? At this point I will be taking 6mg once a week.

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#44

True… everyone is different in absorption and clearing rapamycin.

I think weekly dosing works for me and most people.

Based on Matt Kaeberlein and others who report on cycles/dose, you want to be as close to a zero trough before dosing again.

You want to tamp down MTOR1 for several days catabolic and then let MTOR2 grow for several days anabolic. You need both clearing and growing.

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#45

This month. Member @man_li sent me samples of his zydus 1 mg tablets and everolimus 20 mg pills - from India.

Here’s a picture of what I received.

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On Tuesday, January 13 after no rapamycin for almost 3 months… I took two of the 1 mg tablets and 2.5 hours later had blood drawn for a Labcorp test. This should produce a C-max of 6 ng/mL reading in my blood.

I then took 1 large pill of everolimus 10 mg… and did another blood draw and test 2.5 hours later.

If good stuff this should produce 30 ng/mL in my blood… for a combination of both doses 36 ng/mL.

I am back on rapamycin… tbh… I missed it. I didn’t really feel on point the whole time… more off these last weeks. But, at a much higher dose - double at 12 mg – my typical dose 6 mg weekly.

I will give it 10 days then back to my weekly 6 mg sirolimus.

One obvious side effect of dose is a very noticeable ringing - tinnitus buzz in my ears. Tolerable… but noticeable.

Will have results in about 5 days.

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#46

So I’m really interested in this test you’re doing. Are you getting “blood/sirolimus” levels tested as part of the blood tests you’re doing?

I’m wondering, given that sirolimus and everolimus are extremely similar molecules, whether the blood sirolimus test also will test the everolimus levels?

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#47

Exactly… it’s the same molecule… same test through Labcorp.I will post the results as soon as I get them.

Did zydus…sirolimus first… what we know should work… everolimus after. Physician said its same… like different generic meds would be the same results.

These two blood samples in lavender cap tubes were sent out to be processed. Most of the other blood work was done in town within hours of blood sample taken.

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#48

There’s a separate blood test intended for everolimus. So it’s likely it won’t show up for the sirolimus test.

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#49

Hmmm… well I guess we’ll soon know… if only the zydus shows up at 6 ng/mL… or there abouts on both tests.

However, a significant increase will have to be showing the 10 mg everlimus affect.

Curious to see. Should know by Monday.

Expert opinion:
In comparison to sirolimus, everolimus has higher bioavailability, a shorter terminal half-life, different blood metabolite patterns, the potential to antagonize the negative effects of CNIs on neuronal and kidney cell metabolism (which sirolimus enhances), the ability to stimulate mitochondrial oxidation (which sirolimus inhibits) and to reduce vascular inflammation to a greater extent.
A head-to-head, randomized trial comparing the safety and tolerability of these two mTOR inhibitors in solid organ transplant recipients is merited.

LabCorp test code 716712 (sirolimus whole blood) and 3658513 everolimus
Lavender topped vials

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#50

Hello @man_li

My Labcorp tests are back… and your zydus/ sirolimus product from India results are in… drum roll please🙏

BOOM. It’s good stuff!

I took 2 mg and got a 4.5 ng/mL after 5 hours.
Very solid hit. Typically you get 3 ng/mL per 1 mg. The score of 4.5 is 2.25 per pill. Nice!

The Everolimus sadly did not register.

Unfortunately, the rapamycin/sirolimus Labcorp test is different from Everolimus… but I would suspect its good… because my tinnitus went from slight awareness with 2 mg zydus to major overdrive after the 10 mg Everolimus . Still noticeably ringing 3 days later. Can feel the jolt over my whole body.

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Congratulations!

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#51

:grinning: So happy to know it!

I wonder did you take them with oily food as well? (for better absorption)

Did you always suffer tinnitus side-effect after taking Rapa?

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#52

I took nothing to enhance the zydus… actually was on a fasting stomach due to cholesterol testing… so, yes I imagine you’d definitely get higher numbers with olive oil, fatty meal or Grapefruit Juice a few hours before dose.

Never had tinnitus before starting rapamycin dosing… only noticeable a few hours after dose … lasts for a few days.

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#53

Thank you very much!

I mean did you have tinnitus every time when you took Rapa in the past? Do you know why?

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#54

Yes, after dosing rapamycin my ears ring for several days… then goes away until the next dose.

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#55

That’s weird.

Thanks for your response. :grinning:

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#56

May I know how much weight you are?

Thanks a lot.

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#57

Yes. 190 pounds 6 ft. tall.

Went up an inch taller since on rapamycin. Lol

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#58

Is this a joke or you’re serious? I’ve heard people claims getting a bit taller (more like 1/2 inch) from taking HGH (even some claims of increases where it matters most in men :slight_smile: though I doubt that one) but NEVER from Rapa.

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#59

Let me clarify, I had been just at 6 ft tall my adult height from 30 years up to age 50 years; however, in my early 60’s my height was was 5 ft. 11 in. A loss of about an inch.

I have been on TRT Cypionate 200 mg 1 ml weekly thigh injection for 6-years this month. Grew a lot of muscle - and lost a good bit of fat.

Then, went on rapamycin will be 5-years in August - an early adopter. Lost 20 pounds of visceral fat - muscles became more defined and lean/shredded. Tendons and spine became more flexible.

Last, started HGH 3.5 iu daily injections for thymus rejuvenation, will be 1 year this month. Posture really improved, arm hair grew thicker and longer.

So I am leaner, stronger and tight with significant posture improvement.

Just went up to family medicine cliinic and checked height 5 ft 11 3/4 in.

bmi-chart
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My naked body weight hangs at 188 to 190 pounds. By all accounts my BMI should be 126 – Over weight, but in my DEXA is at 125 normal. Will get a new DEXA in a few weeks and look for changes.

So I am attributing the height gained to loss of weight, increased muscle tone, flexibility and possible HGH pushed up my posture - not crazy - but at almost 68 years 3/4 inch up is great. The trend should be getting shorter.

AI says: nearly 80 percent of people will shrink an inch or two as they age.
Between the ages of 30 and 70, most men lose an inch in height, while most women shrink by about two inches.

This shrinkage typically becomes noticeable around age 40, when people begin to lose about a half an inch of height per decade. After 80 years, both men and women may lose yet another inch.

Here’s to maintaining height - and where it matters, yeah above average there it says. I am in the 1% (you can look it up online if curious). I think rapa, TRT and HGH helps that too. Eat your heart out Bryan Johnson.

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#60

I suspect it is mainly HGH plus exercise. Losing weight actually has a bit of a shrinking effect even on height, at least that’s what I was once told by my doc, more like 1/4 of an inch, nothing major. On HGH though a lot of people report gains in height.

Either way, that’s great you are still about same height at almost 70 as you were in your 20’s. Everybody loses 1 to 1.5 inches by the time they reach 70.

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