A new category of supplements to keep an eye on:
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Tirgonelline content of some food items
| mcg/g | |
|---|---|
| coffee | 2200 |
| chick peas | 350 |
| pea | 280 |
| lentils | 250 |
| rolled oats | 230 |
| alfalfa sprouts | 181 |
| snow pea sprouts | 160 |
Minute 4:20
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Dry powdered wild fenugreek seeds can contain up to 12% of trigonelline
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How many mg or grams per day. (PS 1kg Fenugreek powder in shopping basket ready to go 
)
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Give me time to poke around. But Vincenzo Sorrentino has applied for a patent for a trigonelline supplement.
CLAIMS The invention is claimed as follows:
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A composition consisting essentially of trigonelline or consisting of trigonelline.
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The composition of Claim 1, wherein the composition is formulated for enteral administration.
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The composition of Claim 1 or 2, wherein the composition is selected from the group consisting of a food product, a food supplement, an oral nutritional supplement (ONS), a medical food, and combinations thereof.
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The composition according to any one of preceding claims, wherein at least a portion of trigonelline is isolated.
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The composition according to any one of preceding claims, wherein at least a portion of trigonelline is provided by a plant or algae extract in the composition.
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The composition according to any one of preceding claims, wherein at least a portion of trigonelline is provided by a trigonelline-enriched plant or algae extract in the composition.
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The composition according to any one of preceding claims, wherein the trigonelline is selected from an extract of coffee, fenugreek or algae.
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The composition according to any one of preceding claims, wherein trigonelline is selected from an extract of fenugreek which contains at least about 25% - 50% trigonelline.
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The Composition according to any one of preceding claims, wherein trigonelline is chemically synthesized and which contains at least about 90% trigonelline.
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A method for increasing intracellular nicotinamide adenine dinucleotide (NAD+) in a mammal, the method comprising administering a composition consisting essentially of trigonelline or consisting of trigonelline to the mammal in an amount effective to increase NAD+ biosynthesis in one or more cells of the mammal.
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Thats actually a good sign… if the guy is going through the hassle and cost of of filing a patent (I vaguely recall that the cost is typically around $5K working with IP lawyers in the US for a basic patent), it means he thinks it is worth the investment in time and money.
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Lustgarten took 42 grams of chickpeas prior to his 1/14/24 minute 2:45. That is about 147 mcg (.42 of 350 mcg).
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Unfortunately, you are probably not going to get a large enough dose of trigonelline from a Fenugreek supplement. One Fenugreek extract supplement on Amazon claims to have 2.5% trigonelline in each 500mg. capsule. That would amount to ~12.5 mg. per capsule.
I have tried Fenugreek in the past with no discernible result in lowering blood glucose.
I don’t know how much you would have to take to get the other claimed benefits.
The lowest effect dose in rat studies that I could find was 50mg/kilogram. This would amount to ~ 3.5 grams of trigonelline for a human equivalent dose in a 70 Kg man.
“Overall, the animal research indicates that trigonelline doses of 50-100 mg/kg appear effective at lowering blood glucose in diabetic rodent models.”
Human Equivalent Dose (mg/kg) = Animal dose (mg/kg) x (Animal Km / Human Km)^0.33
Where Km is a correction factor based on body surface area. For a rat, Km is 6, and for a human, Km is 37
Don’t necessarily trust my math. I am old.
Experimental diabetes treated with
trigonelline: effect on b cell and pancreatic
oxidative parameters
https://sci-hub.se/10.1111/j.1472-8206.2011.01022.x
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Chickpeas did not move the needle. Lustgarten tried 24 g of fenugreek. Data will be available in a few weeks.
If the aim is to increase NAD, Lustgarten’s experience shows the best approach is to take 600 mg of nicotinic acid (NAD 67.4); better than 2,000 of NMN (NAD 61.0).
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So at roughly 10% content, then 30 grams daily of fenugreek. Will try . Wish me luck 
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Update: tomorrow starts my experiment:
2 tablespoons of ground fenugreek seeds
2 tablespoons of chlorella
All mixed in almond milk
I have no idea how this will taste, but I think it’ll be yummy. Do I have evidence for this belief/hope? No. But I’ll let you all know if this recipe works.
Might need to add a tablespoon of manaku honey.
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I thought he switched to 60mg since 600mg lowered his epigenetic age. He still got an NAD boost at 60, as I recall.
Where’d you get the idea for chlorella? The mention of algae in the patent?
The patent actually states Laminariaceae - kelp.
SUMMARY
[004] The present disclosure provides a composition consisting essentially of trigonelline or consisting of trigonelline. In some embodiments, at least a portion of the trigonelline is provided by a plant extract in the composition, such as one or more of a coffee extract, a hemp extract, a pumpkin seed extract and/or a fenugreek seed extract, for example a plant extract enriched in trigonelline.
[005] In a preferred embodiment, at least a portion of trigonelline is provided from a fenugreek extract. [006] In some embodiments, at least a portion of the trigonelline is provided from an algae source, for example, a Laminariaceae extract.
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Oh sorry for not being clear. The Chlorella is just something I bought a while ago and been trying to figure out a way to incorporate into my daily regime. The chlorella is for the spermidine content (approximately 300ppm or 0.06%). So two tablespoons (30 grams) should give me roughly 18mg of spermidine per day (I think 
)
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I get the spermidine from aged cheddar. It also has the C15 fat, so two birds…
If I were to go vegan, I would prefer mushrooms…
The highest content of spermidine was found in the fruiting bodies of unprocessed brown and white mushrooms (367.22 ± 14.19 and 266.47 ± 13.38 mg/kg, respectively).
That is 3.67 mg per 10 grams, or 11.01 mg per 30 grams. One needs to take more, but it is more palatable, and more sustainable long-term.
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UPDATE
That was one of the most disgusting things I have ever drunk. Trying to squeeze 4 tablespoons of powder into a single drink results in a thick sludge. Which would have been okay if that sludge was sweet like melted chocolate or salted caramel.
This was NOT the case.
It tasted like I was drinking bitter curry flavoured seaweed.
It was also luminous green!!! OMG
I imagine 1 in a billion humans will find this tasty. I am not one of those 8 people.
Two lessons.
- I will only try less powder maybe over two or three drinks.
- I will add something sweet. I am thinking Treehalose,
Will update again.
Please, if you have a healthy recipe proposal - I am all ears 
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I think I will try mushrooms once I’ve worked my way through this kilogram bag of chlorella
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Thank you @RapAdmin for alerting me to this compound in Fenugreek.
As I have been open about on this forum, I have Multiple Sclerosis.
Interesting that Trigonelline is also helpful in neurology:
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Often fenugreek seed powder is made into a “tea” by straining it, but who knows how much trigonelline is in the tea, and does it depend on the fineness of the straining? I saw one estimate that a serving of prepared coffee contains about 5mg, but likely that varies by brewing method.
I haven’t found any good source for higher dosage supplementation.
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Renue by Science has this