Another strategy for increasing polyamine availability consists in administering yogurt containing the precursor arginine and Bifidobacterium animalis subsp. lactis, which generates spermidine in the intestinal tract. This synbiotic combination of prebiotic and probiotic caused increased putrescine and spermidine levels in blood, decreased heart rates, improved endothelial function and improvements in the blood lipid profile199,200. Moreover, spermidine supplementation reportedly prolongs the anagen phase of hair follicles in a randomized, placebo-controlled, double-blind trial201, confirming similar results from preclinical studies148,202.
They call it by itās proper name āBifidobacterium anamalis subsp. lactisā and apparently arginine is a precursor and that is what turns into spermadine. I was thinking of adding it to the yogurt or chewing up a tablet. Have not tried that yet.
This is the japanese study on humans where they gave the yogurt to the test group and not to the placebo and they got all kinds of great results. Probably has something to do with the popularity of this combo in Japan.
They really didnāt give that much yogurt, it was a 100 gram mix of the placebo yogurt and bb12. They used as a placebo something that would die in the stomach.
Since Spermadine and Rapo are both MTOR inhibitors does it make sense to take both? Iām thinking out loud if a good protocol might be 12 weeks of Rapo followed by 12 weeks of Spermadine, repeat
I doubt that you can place spermidine in the same classification with regard to mTOR inhibition, as rapamycin.
I suspect its a very mild inhibitor (I have not read all the research lately, but given the moderate at best results researchers have been suggesting with spermidine, that is my feeling). So I layer on spermidine right now just as a possibly beneficial supplement that may or may not be worth the price.
So my focus is on optimizing rapamycin use as its the most proven longevity drug and the most powerful, and all supplements (with the exception of Astaxanthin, with its 12% median lifespan improvement) playing a relatively low priority/importance for longevity in my estimation.
Save your money if trying to supplement with spermidine :
High-Dose Spermidine Supplementation Does Not Increase Spermidine Levels in Blood Plasma and Saliva of Healthy Adults: A Randomized Placebo-Controlled Pharmacokinetic and Metabolomic Study
Yes - this was the same result that Richard Miller reported at the NIA ITP program when they looked at it and tested it for 6 months in mice. They couldnāt detect increased levels of spermidine in the blood. This leaves spermidine as a large question mark in my view.
Are we sure that its main mechanism of action is via the blood?
For instance, many believe much/most/all of acarboseās longevity comes via how it helps remodel the gut and not primarily via the glucose modulation per se.
Never heard/seen Richard Miller address the equivalent family of points from Bicep:
Relatedly saw Mike Lustgarden just re tweet:
ā Out now! Over a century ago, Elie Metchnikov proposed that #aging is caused by increasing #intestine permeability and toxic #microbiota into our bodies. In our last paper provides evidence for this idea.ā
For me as well. Back to the drawing board with Spermidine. Ive been supplementing with Wheat Germ and Iāve only felt that it was providing excess calories.
The CEO Grace Lin Xu responded on Facebook to my enquiry if they are still taking orders and she said " We are out of stock now tks for the interests !".
Since I did not specify what item I was interested in, it looks like the company may have suspended operations during 2022.
Looks like using a Yogurt maker to boost the yield from the 1 billion (approx) BB-12 in TruBiotics is the only way to get a decent amount of BB-12.
JuanDaw and @Bicep, After the NIA ITP found no increase in mice blood levels of spermidine following 6 months of feeding with the recommended dosing of spermidine, I lost most of my enthusiasm for spermidine.
So, at least in my mind, the previous research showing spermidine benefits is redeemed and again seems credible. It seems likely that the benefits were simply a result of the spermidine metabolite spermine.
I also note that Bryan Johnson supplements daily with spermidine.
Are you still making the BB12 yogurt? If so, are you making it from capsules? It was last December when this was being discussed in the topic Spermidine delays aging in Humans .
I have a yogurt maker, an Instapot, and capsules containing Bifidobacterium, BB-12. Iām ready.
Yes, I have some every day. I use the Trubiotic capsules. 1 capsule in 1 quart of half and half. I add arginine, but just 750 mg. Thatās what turns into spermadine. I should buy in bulk and use more since itās cheap. I also add a tbs of flax oil because they convert the linoleic acid into CLA. Miraculous. I use inulin as feed since you need it anyway and it does not get used up. Can cause gas, beware.
I go 98.5 degrees for 24 hours. 36 will make curds and whey, which is not bad, but not what you want.