Sharing the results of a six-month self-experiment I ran to try to repair long-standing gut issues. I’m not making any claims—just putting this out there in case it’s useful. The data is messy, I’m not a mouse. I don’t live in a sterile lab and I’m aware of the limitations. Still, the directional signal seems meaningful.
BACKGROUND
I’ve had gut issues ever since two rounds of Cipro about six years ago. Shortly afterward I developed gastritis, and since then I’ve had recurring stomach discomfort, with monthly bouts of nausea and headaches. The episodes are bad enough that I’ll sometimes sleep half the day. I thought histamine intolerance might be the culprit, but I could never pin it down.
I came across Trulacta via Bryan Johnson, who posted about its potential to slow biological age:
I wasn’t particularly interested in the aging claim. What caught my attention was the idea that the components of human breast milk might help with microbial rebalancing and pathogen reduction.
I have a: “young signaling into old organisms” theory. That includes stem cells, exosomes, FMT, and in this case, lysed cell components and postbiotic factors. Trulacta fits that model.
PROTOCOL
· Took Trulacta twice daily for 6 months
· Diet and exercise remained stable during that time
· Did a Viome test pre-intervention and **another at the 6-month mark
· Used Claude and ChatGPT to help interpret the results, and asked Claude to compare against centenarian microbiome signatures (with appropriate skepticism)
INITIAL REACTION
First dose: within an hour, I experienced headache, nausea, and nasal congestion, which I’ve learned is a Herxheimer reaction. (I wasn’t expecting this at all) My HRV also spiked to 55ms that night—my highest ever. Could be coincidence or parasympathetic rebound from microbial die-off. It normalized the next day.
QUANTITATIVE RESULTS
Here’s a simplified summary of what changed, based on Viome data and post-analysis.
|Metric. |Before|After|% Change|
|Gut Diversity Score. |81|94|+16%|
|Bifidobacterium species. |9|15+|+67%|
|Total Beneficial Species|~45|~65|+44%|
|Major Pathogens. |8|3|-63%|
|Oral Bacteria (in gut). |15+|10+|-33%|
|Plant Viruses. |4|3|-25%|
Some of the most relevant Viome categories—like oxalate metabolism and butyrate production—also improved modestly. The rest were either flat or slightly improved.
For fun, I asked Claude to compare my microbial profile to known traits in centenarian microbiomes. I know it’s not very scientific, but it was interesting.
CENTENARIAN MARKERS:
· High Bifidobacterium diversity 
· Presence of Akkermansia muciniphila
· SCFA producers maintained (e.g., Faecalibacterium)
· Christensenella increased from 1 to 2 species
· Pathogen load significantly reduced
· Moderate improvement in Proteobacteria
· Enhanced gut barrier species
According to the model, I moved from 4/8 markers aligned with centenarians to 7/8—an increase from 50% to 87.5% alignment. Again, not perfect, but the direction is encouraging.
Claude also generated a “Weighted Health Index” using simple scoring based on the Viome data:
· Before: 46.5 / 100 (Poor–Fair)
· After: 73.75 / 100 (Good–Very Good)
· Improvement: +58.6%
Weighting was based on relative importance of pathogens, beneficial strains, diversity, symptom resolution, etc. I know that’s also subjective and we can’t trust anything AI tells us, but I found it helpful as a summary.
SYMPTOM RESOLUTION
·Headaches and nausea are gone.
This was the biggest surprise. I had written these off as either unrelated or something I’d have to live with. They haven’t come back since Trulacta use.
·Stomach discomfort improved but not resolved.
Still have dysbiosis. A couple of new strains popped up post-intervention. Likely from food exposure. (I’m not sure how to solve this)
I now suspect I might have hypochlorhydria. Gastritis can damage parietal cells and reduce stomach acid production can impair digestion and allow oral bacterial migration.
So now I’m now adding digestive bitters and HCl supplements, which makes things even more messy, but my goal is to fix the problem not to publish a study.
CONCLUSION
Trulacta does what it claims. It’s improved my gut diversity, expanded my beneficial bacteria and reduced pathogen load. It also resolved some long-standing symptoms, but it’s not a silver bullet.
It’s the most meaningful shift I’ve seen after years of trying probiotics, diet changes, etc. I clearly have a ways to go, but I’m pleased to see an improvement. I’m glad I tested, I’d have had no idea otherwise, so worth the investment.
For Phase 2 I’m now adding THAENA to the stack , a postbiotic derived from sterilized healthy human stool. No live bacteria—just metabolites. It’s the closest thing to an FMT without a transplant.
Thaena also outperformed Rapamycin for lifespan results in Nematode worms. (not making any claims, just interesting)
Only two weeks in, but I plan to test again in another six months. Will report back if there’s interest.
I’d love to see if anyone can replicate my results.
Trudiagnostic is also running a trial on Trulacta’s effects on epigenetic age (Florida-based):
https://ichgcp.net/clinical-trials-registry/nct05297097
I hope this is helpful. Happy to answer questions.