A short one here. Last year I made some measurements (4 indeed!) which I posted here already:
Blood concentration of rapamycin (N of 1 dosage, model and biomarkers)
Last month I did the same and the results are very different.
Last year followed by now:
The first difference is obviously the half life which was 83h and is now a more standard 65h.
That was expected because Berberine inhibits the activity of several cytochrome P450 (CYP450) enzymes in the liver (specifically CYP2D6, CYP2C9, and CYP3A4).
What was not expected is the much lower level of rapamycin even though those enzymes are inhibited.
My current rapa is Dr Reddy’s which I got from CVS while my previous one was from GetHealthspan but I don’t remember which brand.
Just a reminder for people to test their rapamycin levels as there is a lot of individual as well as brand variations.
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FWIW, my 50 hour sirolimus blood levels were 5.6 ng/mL on the Biocon brand and 4.4 ng/mL on the Zydus brand. Both measured by LabCorp. Same manner of taking (on an empty stomach after an overnight fast and no food for the subsequent 3-4 hours), same diet, exercise, supplement regimen, but on the Zydus I added bempedoic acid 180 mg/day, and escalated my empagliflozin dose from 12.5 mg/day to 25 mg/day.
I intend to take more tests with different time periods. But I have also gone back to the Biocon brand for now.
What was the dose for those levels?
Oops, sorry! Essential info. 6mg once a week. Every Saturday morning.
That’s similar to my levels at 50h except that I use 12mg and not 6.
It could be because of the accumulation over time as you still have some remaining after 1 week.
You should measure at least 2 points to compute the half life.
Yes, I measured 50 hours from Saturday to Monday. I’m planning on measuring Monday 50 hours and Wednesday 98-100 hours, but my next test is going to be Friday 144 hours (i.e. 24 hours before my next dose).
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