For some reason this seems very interesting to me.
It is precisely because the current view is that the gut bacteria is very important.
This is a very contrarian intervention. Surely Peter Diamandis is familiar with the gut microbiome talk and trend. And he takes it despite it. Talk about going against the grain.
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I’ve heard it’s common in China to take antibiotics (like in a drop-in clinic with IV), for overall wellness, is this true? Not saying it’s bad or good, just curious.
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It’s possible that at this low dose weekly it improves the microbiome and reduces the microbial burden. It could act as a selective herbicide does for the farmer, to hurt the bad guys and make it a little easier for the good.
I’m not an expert at this, but it’s not the craziest thing I’ve seen. Farmers (whether right or wrong) have for years fed tetracycline because it makes the animals healthier and makes money. I don’t know if they still do, I’m not in that business any more.
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https://www.costco.com/cmpps?drugIdentifierParam=55698435705&drugNameParam=Doxycycline+Hyclate
Doxycycline, 20 mg, 60 tabs, $18.99.
Four studies reported on the effect of doxycycline on faecal microbiota, one at suboptimal dosage (20 mg for 9 months) in patients with periodontitis57 and two at usual dose 100–150 mg for 7–10 days, although one also with a probiotic,56 58 and another at low dose (40 mg for 16 weeks),59 as described in table 2. Doxycycline treatment did not significantly affect counts of total anaerobic bacteria, candida, total enterics, Staphylococcus or doxycycline-resistant bacteria recovered at any of the sample periods and did not result in the development of multi-antibiotic resistance.57 Matto et al 58 specifically evaluated the influence of doxycycline therapy on the composition and antibiotic susceptibility of intestinal Bifidobacteria in nine subjects while they were also taking a probiotic and compared these to adults consuming only probiotics. A marked decrease in diversity of Bifidobacterium populations was observed during doxycycline therapy. Tetracycline-resistant Bifidobacterium isolates were more commonly detected in the antibiotic group than in the control group, thus increasing the pool of resistant commensal bacteria in the intestine.
In summary, doxycycline interferes with a microorganism’s ability to manufacture proteins. At suboptimal dosage (20 mg per day), it has little effect on the gut microbiota with the exception of enterococci and E. coli .
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Yes, at 20mg it seems to be an AINO (Antibiotic In Name Only) and would seem to be safe even long-term.
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@Agetron you still doing Doxycycline 200mg with your Rapa dose 100 day and 100 night or all together? I am going to do this too since I now have a bionic hip. Figure it should negate the risks of rogue bacteria inhabiting my prosthesis.
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Changes in Gut Microbiota Induced by Doxycycline …
by I Robles-Vera · 2021 · Cited by 6 — Doxycycline Treatment Improved Intestinal Integrity, Colonic Inflammation and Reduced Endotoxemia and Plasma Noradrenaline in DOCA-Salt Rats.
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Yes, I still take 200 mg on the day of my rapamycin dose.Yes, a 100 mg pill in morning and 100 mg at bedtime.
Pretty healthy… skin heals rapidly when injured. Open cut to gone in 5-7 days.
If you at this subclinical dose can see an increase in doxy-resistant bacteria and a decrease in friendly bífidus bacteria, I think it bodes bad for chronicle use at therapeutic dosage. It’s prescribed for youngster with severe Acne, but I don’t see it as proof for that it’s without side effects. Maximal treatment length against acne should be 3-4 months because the risk of developing resistente bacteria.
Doctors are probably prescribing doxy quite frivolously, because there already are a lot of of resistant bacteria circulating, knowing that they have heavier (as still effective broad spectrum) weapons in their arsenal.
I for sure know that i won’t volunteer to become a vessel for growing these resistente bacteria in my gut.
But each to their own. 
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I have been on the 6mg Rapa plus 100mg Doxcycline regime for about 4 months (almost immediately once I learnt about this).
The reason I was so quick to jump on this was twofold (I also have MS):
Synopsis: Addition of doxycycline to interferon-beta therapy in patients with relapsing-remitting multiple sclerosis might improve control of inflammatory lesions.
The rapamycin + doxycycline combination decreased tumour proliferation in about 2/3rd of the investigated cell lines. The continuous treatment reduced tumour growth significantly both in vivo and in vitro.
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I should have thought of this sooner. About a third of my femur plus my entire knee are mostly stainless steel (I had chondrosarcoma) and a big concern is infection which - if it gets to the implant - is Very Bad.
Thanks for the insight!
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Affordable Rifaximin/Rapamycin, Rybelsus,SIBO Discussions
Point by point analysis by Richard.
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It sounds like he’s dropped the doxycycline he was taking with rapamycin.
I also found this interesting:
And his current protocol:
Longevity Practices_27Nov23.pdf (658.2 KB)
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It makes me wonder if a alternating schedule on a twice weekly dosing regimen could mitigate the potential for problems. My thought was 50 mg twice a week for Doxy, but I don’t know minocycline well enough to know the dosing. Also, how hard is it to get minocycline? It’s not likely the traditional medical system would be willing to prescribe it. I wonder if it’s available from international sources? I see that it’s cheaper through a veterinary sources by a factor of 10!
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Minocycline is commonly prescribed in the US, I write scripts for teens with bad acne who get stomach upset with doxycycline. It has neuroprotective features and decreases inflammation in the central nervous system. Super inexpensive especially if you use Amazon medical and pharmacy.
Just be careful with long term use of minocycline because unlike doxy, mino can cause a permanent blue/gray discoloration of the teeth and gums. It can also cause dark navy-blue patches in the skin. I’ve seen this a number of times over the years, usually patients who come to derm in a panic and have been on minocycline for years, prescribed by a provider who didn’t know any better. This is almost never seen with short term use but gets more common the longer it is taken.
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I have taken it BID for years, and acne, still at 64, but I believe you, just have not experienced it pesonally.
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Wow you are fortunate. At least you’ll know what it is immediately if it ever happens. It can be reversible after cessation of minocycline in some cases, but not always.
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Here’s an update on Peter’s protocol. Posting this impartially. I’m a really interesting using Immuna from Immunis and the Throne Immune reprogramming.
Definitely something I’d like to get my hands on.
PROTOCOL AS FOLLOWS:
- PEMF (Pulsed Electromagnetic Field) therapy runs automatically from 5:00-5:30am while I’m still in bed; and then again at 9:30pm when I’m going to sleep.
Morning Routine (5:30am - 7:00am)
- Red Light Therapy (30 min): I use three devices simultaneously: a PlatinumLED BIOMAX 900 panel for my body (20 minutes), a laser cap for hair growth (10 minutes), and a red-light mouthpiece for gum and teeth health (10 minutes). David and I both swear by red light: it reduces inflammation and promotes healthy skin and tissue repair.
- Meditation: 15 minutes using an Ohm device. My meditation focus every morning is on GRATITUDE and Optimism. The data is clear: optimists live 15% longer than pessimists. Your mindset is the most underrated longevity lever.
- Workout: 40 minutes of weight training, targeting upper and lower body. Muscle mass is one of the strongest predictors of longevity. My goal: resistance training 5 days a week, 1g protein per pound of bodyweight, and 5g of creatine daily.
Morning Peptides
- CJC-1295: 5 days/week, a growth hormone-releasing hormone analog for tissue repair and recovery.
- SS-31 (Elamipretide): Sunday & Wednesday. Targets and repairs mitochondria directly.
- MOTC: Sunday, Wednesday, Friday. a mitochondria-derived peptide that enhances metabolic function.
- Tirzepatide (microdose): Sunday only. It’s a next-gen GLP-1 receptor agonist. David and I discussed this at the Abundance Summit. Beyond weight loss, GLP-1s are showing remarkable benefits for the heart and brain. We’re both watching this space closely.
Evening Peptides
- Sermorelin: 5 days/week. A peptide used to stimulate the pituitary gland to produce and release more growth hormone.
- BPC-157: Sunday thru Thursday. A peptide used to accelerate healing of tendons, ligaments, muscles, and gut tissue.
- DSIP (Delta Sleep-Inducing Peptide): A naturally occurring neuropeptide that, when administered intranasally, is reported to promote slow-wave (deep) sleep and improve sleep quality.
My Supplement Stack
As I’ve mentioned before, I take a stack of over 60 supplements per day divided into a wake-up, AM, Lunchtime, PM and Bedtime pill packs. Without listing everything, I want to say I mirror nearly 100% of what David listed above.
As always, I have to say you should discuss your supplements and meds with you physician . Having said that, the following supplements are, in my humble opinion, useful for most everyone, and a few I will list in addition to those listed by Dr. Sinclair:
- Creatine 5 grams every day: Fuels muscles, boosts strength and power output.
- Coq10 with PQQ: Cellular Energy and support: mitochondria support.
- Urolithin A: Mitochondria Support.
- N-Acetyl Cysteine: Precursor to glutathione (the body’s master antioxidant).
- Magnesium Glycinate & Magnesium Threonate: Brain and Sleep support.
Therapeutics
- Infrared Sauna: 3x per week, 20-30 minutes. I use a “Healing Sauna ,” which is super portable and keeps my head and arms free to do work. David and I agree: heat stress activates your body’s “adversity mode,” triggering longevity pathways. Your air-conditioned, comfortable life is actually working against you.
- Exosomes, Immuna and Throne Biosciences: Through Fountain Life , under the care of my physicians, I’ve used three different rejuvenation protocols, specifically: (i) IV exosomes (Kimera Labs ); (ii) Immuna, an experimental protocol under phase-2 FDA trial from Immunis ; and (iii) Stem Cell Educator Therapy by Throne Biosciences : a one-time, dialysis-like treatment that uses CB-SC stem cells derived from human umbilical cord blood to “re-educate” and reset the immune system.
My Skin Protocol. This Is Where the Science Gets Exciting
Here’s something I’ve become increasingly passionate about: your skin is not just cosmetic. It’s your body’s largest organ and a major driver of systemic aging.
Both morning and evening, I apply OneSkin’s OS-01 FACE and BODY formulations.
Why? Because the science behind OS-01 is some of the most rigorous I’ve seen in the longevity space. OneSkin was founded by four female PhDs from Brazil who asked a simple question: What if you could eliminate the senescent “zombie” cells in your skin that drive aging?
They screened roughly 1,000 peptide sequences and found one, OS-01, that does exactly that.
Here’s what the peer-reviewed data shows:
- Reduces senescent cell burden by up to 50% in skin tissue models.
- Decreases skin’s biological age by 2.5 years in just 5 days of treatment, measured by MolClock, the first skin-specific molecular clock.
- Increases epidermal thickness and supports collagen biosynthesis the key building block of younger skin.
- A new 2025 pilot study published in the Journal of Cosmetic Dermatology found that OS-01 BODY, their body moisturizer with the OS-01 PeptideTM, increased skin’s barrier strength , leading to a reduced systemic inflammatory marker (IL-8), even though the peptide never enters the bloodstream.
That’s not skincare, that’s longevity science applied through your body’s largest organ.
And this actually makes a lot of sense when you consider that skin is your body’s first line of defense against external aggressors, and the fact that we live in a toxic soup these days.
David’s work has taught us that senescent cells are among the primary accelerators of aging. His lab uses gene therapies to target them systemically. OneSkin is doing something complementary: targeting senescent cell accumulation in the skin with a topical peptide, and the downstream effects on systemic health are remarkable.
I apply OS-01 FACE morning and evening, and OS-01 BODY every morning. It’s part of my protocol because the science warrants it.
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