The video said their skin is thick and they have to use high doses to penetrate. Ingestion should work at lower doses I’d think.
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One conclusion I’ve come to is that taking rapamycin pills probably requires a lower dose than what is used in animal trials, be it in mice or worms.
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Why lower dose than mice that get it from their feed? I agree dosing from nematode to human is basically impossible, the video said as much have to use a mammal.
Krister, here is another nematode researcher that you may want to talk to. He has a company that is also doing nematode longevity research (perhaps a little competitive with Ora Biomedical)…
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There are updates in the Ora Million Molecule Challenge Data Hub!, mixtures and some more compounds have been included.
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GIven all the stress about Omega 6 it is interesting to see Linoleic Acid in the leaderboard.
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I was not surprised to see that. Ive seen lots of studies about how it doesnt cause inflammation
And it does promote longevity and heart health.
Omega 3 is still better and you want to take enough to keep omega 6 ratio not too bad to be safe.
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I got excited by the new leaderboard and sponsored a bunch:
Berberine HCL
Epigallocatechin gallate (EGCG)
Sulforaphane
Tramiprosate
Glycine
Edit: Has anyone started trying to combine 2 of the top candidates? I think other amino acids like Serine would also be good to test. It seems taurine is not that good since with rapamycin it has no net effect, and rapamycin was tested individually to have no effect. Seeing if leucine or iso-leucine are negative would also be interesting
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I’ve just sponsored:
- Semaglutide: https://www.caymanchem.com/product/40231/semaglutide
- Urolithin A: https://www.medchemexpress.com/Urolithin-A.html
- Obicetrapib: Obicetrapib |CAS:866399-87-3 Probechem Biochemicals
- R-(-)-deprenyl hydrochloride (aka selegiline): https://www.sigmaaldrich.com/US/en/product/sigma/m003
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https://twitter.com/OraBiomedical/status/1781331548274647431?t=t9KrSXFpnhY3VZ7IZGjZ3Q&s=19
Guys you can test combinations for free they are sponsoring them don’t miss it
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Have you seen the result of the nilvadipine?
It appears in the intervention browser but not in the compiled data.
(I have not sponsored it)
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How were your concentrations/doses selected? 50 uM seems extraordinarily high for any drug. While SGLT may not be its mechanism of longevity enhancement, empagliflozin would provide 80% inhibition of SGLT-2 at about 12 nM. I would be concerned 50000 nM ( = 50 uM) would have nonspecific toxicities.
When people do in vitro testing, 10 uM is often used as a default real high concentration to see if anything happens. But unless you’re looking at the liver or a few other edge cases, biologically relevant concentrations of drugs are usually below 1 uM.
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Can you please ask them? (on Twitter or by email) I don’t know enough about this issue but they said once that before testing something they look at a dose response with 3 different doses.
Sure. I’ll post if/when I get a response to my email.
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I asked them and they said default they test at the maximum concentration that dissolves since worms have thick skin and they want to make sure it has an affect. Then they can try lower doses later. It doesnt seem they think too high doses is a big deal. For berberine or tumeric they cant get enough to dissolve to match other papers
Ok, this is funny to me. @AnUser accused me of believing in magic crystals and shungite:
https://www.amazon.com/Elite-Shungite-Stone-Purification-Mineral/dp/B071ZZ93FK/
Shungite is a magic crystal that is said to purify water or if you wear it around your neck it will keep away bad vibes. It is almost pure carbon and the form is mostly fullerines or buckyballs. So I wanted to have shungite tested in the Million molecule challenge. Emailed them and got this back:
Thank you for your interest in the Million Molecule Challenge and Ora Biomedical!
** We have not tested shungite. For safety and liability, we must source compounds from reputable research grade chemical suppliers.**
** That said, in my quick literature search, fullerene may be a suitable replacement. That is offered by chemical suppliers. To run that test, we would need to charge for compound purchase and shipping. An estimate for a single dose test would be around $400.**
** Let me know if you are interested and I can draft a custom contract.**
So the price is a little higher than I wanted, also not testing shungite (obviously magic for some reason) but fullerines. I have no idea how much to use or whether to mix it in the feed or just use it for some form of bedding to ward off bad vibes.
Does anybody have a good idea here?
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What are the odds, some random 2 billion year old rock I mentioned for fun?
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Yeah, I knew about c-60 and couldn’t find it on the list either. I’m surprised they didn’t have it on there. Sarah Westall sells it on her program all the time. Supposed to cure all kinds of stuff. LOL.
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Yes, it is a little pricey even if you make your own.
Frome SES Research:
"SINGLE BOTTLE ESS60 AVOCADO OIL
ESS60 - Carbon 60, 99.99% in Avocado Oil Extra Virgin, Organic, 120ml" $79.95"
From “https://www.sesres.com/carbon-60-supplements/c60-in-avocado-oil/”
I agree with @Bicept, that avocado oil is your best bet to avoid digestive tract problems.
I tried C60 in olive oil several years ago but felt no subjective benefits, I didn’t notice any effect on my blood work so I didn’t continue taking it.
I’m sorry but there is little proof that this does anything in humans.
FWIW: Here are two YouTube videos showing step-by-step how to make your own C60 Olive Oil.
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