Steve what’s happening here?
Decision has been delayed until Sept 30, we’ll see.
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Sept 30 the FDA decided that NMN is a dietary supplement.
Good news for many of us.
NMN and NR increased our telomere length consistently over 5 tests in 4 years. Most will say that is irrelevant to aging but it may be 
my interest in Telo length stems from how that happens, and it requires the enzyme telomerase to do that.
Telomerase has other health related benefits so I consider longer telomeres indicate a healthy telomerase production system.
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Seems mixed. There will be less incentives to do more human clinical trials and even studies on other animals now.
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This your N=1 data or from a study?
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Our personal results using Trudiagnostic tests N=2
There have been about 18 human clinical studies specifically on NMN supplements, with additional preclinical animal and in vitro studies overall.
Even a dietary supplement, if marketed correctly, can support the cost of studies.
There are over 35 clinical studies published on Nicotinamide Riboside (NR) as of 2025.
Most are small scale and short duration.
Many people use these 2 compounds, we alternate, a couple months with NR and a couple months with NMN
do the studies on NR and NMN indicate telomere lengthening.pdf (159.5 KB)
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Cool that you have so many measurements with them - how did your other metrics and Pace change with NMN?
Do the epigenetically predicted telomere lengths correlate well with actual measured ones? Believe epigenetically predicted like HbA1c, etc were quite bad?
Cool that you have so many measurements with them - how did your other metrics and Pace change with
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Ran both NMN and NR supplementation for several months. No subjective response whatsoever. However, I still believe NAD+ optimization has merit.
My current protocol (both before sleep):
Niacin 50mg - NAD+ precursor, promotes prostaglandin synthesis (sleep-supportive), favorable lipid effects. Dose well below the threshold associated with 4PY accumulation and cardiovascular risk in recent literature.
Apigenin 50mg (on top to chamomile tea) - CD38 inhibitor, reduces NAD+ degradation rate.
Targeting both NAD+ synthesis (precursor) and degradation (CD38 inhibition) simultaneously. Add melatonine to this stack - and you have never slept better. Whether it’s the prostaglandin support from niacin, the anxiolytic effects of apigenin, or actual NAD+ optimization…
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My Pace decreased significantly 1.2 to 0.89 BUT I don’t attribute that progress entirely to GlyNAC. I was also doing some intense clearing of senescent cells for a couple years before I started GlyNAC as that is what go me doing the Tru tests. Then about 2 years in I added GlyNAC.
Also the last test I had done was also 1 year into Rapamycin
I doubt the GlyNAC had that much effect on my Pace but who knows, when one is throwing the kitchen sink at increasing health span.
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Tryptophan is an important actor in the NAD de novo pathway.
About 2 weeks ago we added 1000mg just before bed, not specifically for sleep as we are both excellent sleepers, just did not want the Trypto to make us sleepy during the day.
Tryptophan is an important actor in NAD production.pdf (172.9 KB)
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Thank you, @Steve_Combi, was unaware about this pathway. Seems not so potent acc. to source below, but can be taken into account
- The U.S. Office of Dietary Supplements (ODS) “Niacin — Health Professional Fact Sheet” states:
“The most commonly used estimate of efficiency for tryptophan conversion to NAD is 1:60 (i.e., 1 mg niacin [NAD] from 60 mg tryptophan).”
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that doesn’t make much sense to me, niacin is not NAD+? is there something missing?
In conclusion, accounting for pathway losses, 1 g of tryptophan yields about 10-12 mg of NAD+ via the de novo pathway in humans, reflecting the complex regulation and metabolic demands in the body.
I use tryptophan as a “back up” to ensure that the NAD+ levels are supported through various pathways. One of the reason I switch back and forth from NR and NMN.
Estimate NAD+ yield from 1 g tryptophan accounting.pdf (157.3 KB)
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Sorry Steve - I thought we were talking about NMN?
Got my wires crossed, yes to NMN as providing a potential Pace improvement.
But I think the senolytic program may have had more influence.
The reason I think that is 4 other people followed a 7 month protocol on it (no NMN or NR) and every one had an improvement in their TruDiagnostic scores, including Pace.
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It’s wishful thinking to expect such low doses of apigenin to have significant effects on NAD+ levels. It’s oral bioavailability is far too low for that. I think it’s misleading to promote it as a supplement to increase NAD+ levels IMO.
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In part yes, but it’s mainly a matter of bioavailability. Apigenin is heavily metabolized in the intestines and by the liver in rodents and humans. In mice, this results in very low bioavailability, in humans it’s even worse, since they metabolize it even more strongly.
IIRC, the main study that found that apigenin inhibited CD38 in vivo and created interest in apigenin as a means to inhibit CD38 was a mouse study that use very high doses given intraperitoneally. The intraperitoneal route bypasses the intestines and liver. The mice would need a lot higher oral doses to get the same amount in your blood as they got with the intraperitoneal injection.
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There is a case study about a patient drinking parsley juice daily. The levels of sirolimus increased dramatically. Was it an effect of the apigenin in the parsley? Or something else in the parsley?
And starting at 1.25.50 this video digs deep into the NMN and NR question. Including strongly advising against intrevenous NAD at 1,52,24
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It’s hard to say. Apigenin has some CYP3A4 inhibitory activity so it could be the apigenin. However it could also be something else in the parsley, I’m not sure. Characterization of the CYP3A4 Enzyme Inhibition Potential of Selected Flavonoids - PubMed
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I always wonder why a poorly studied compound like apigenin is promoted and consumed as a NAD+ booster when there compounds like NMN and NR that have significantly more human studies showing that these 2 do increase NAD+ and the doses that work.
Reinventing the wheel is not my thing. So I alternate between NR and NMN, generally 3 month cycles.
And I’ve seen my telomeres increase consistently via TrueDiagnostic testing with 5 tests over 4 years. Should have my TD test result #5 in a few weeks.
And the whole hype around NAD+ IV’s that don’t work.
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