Perhaps a good reason to pair ergothionine with rapamycin:
Ergothioneine Protects Against UV-Induced Oxidative Stress Through the PI3K/AKT/Nrf2 Signaling Pathway
Background: Ergothioneine (EGT) is an antioxidant, which could be detected in human tissues, and human skin cells could utilize EGT and play an anti-oxidative role in keratinocytes. And in this study we are going to elucidate whether EGT could protect the skin from photoaging by Ultraviolet (UV) exposure in mice and its molecule pathway. Methods: Histological analysis was performed for evaluating the skin structure change. Malondialdehyde (MDA) and superoxide dismutase (SOD) levels were measured with biological assay for evaluating oxidative and antioxidative ability of skin exposed to UV light. And the level of marker molecules in mouse skin were detected by hydroxyproline (Hyp) assay, immunohistochemical analysis, Western blot, and quantitative real-time PCR (qRT-PCR). The markers of skin aging and cell death were tested by cell culture and treatment, Western blot and qRT-PCR. Results: EGT decreased the levels of inflammatory factors induced by UV exposure in mouse skin. MDA and SOD activity detection showed that EGT decreased MDA levels, increased SOD activity, and upregulated PI3K/Akt/Nrf2 signals in mouse skin exposed to UV, which further activated Nrf2 in the nucleus and enhanced the expression of Nrf2 target genes. In the cell model, we revealed that EGT could inhibit the increase in senescence-associated β-galactosidase-positive cells and p16 and γ-H2A.X positive cells induced by etoposide and activate PI3K/Akt/Nrf2 signaling. Moreover, a PI3K inhibitor blocked EGT protection against etoposide-induced cell death. Conclusion: The study showed EGT may play an important protective role against cell damage or death through the PI3K/Akt/Nrf2 signaling pathway in skin.
Is there a blood test that the public can use for Ergothionine? I should get a baseline. It rained again and I got another approx 40 lb flush. I’m doing batches and have like 10 quarts of powder. I’m going to take a massive dose and see what happens.
Also, the document below provides some good information but generally ergothionine seems to be early in its research (not a lot done so far from the sounds of it).
Thirty-three young male and female C-57 black mice were given approximately 300 μg of calcium pantothenate daily in drinking water. Forty-one control mice did not receive the vitamin supplement. 2) The mean life span for the mice given supplementary calcium pantothenate was 653.1 days and that for the control mice was 549.8 days. The statistical difference between the 2 groups is P = 0.05 (T test) 0.01 (U test). 3) The mice which received the vitamin supplement maintained slightly greater body weight after they were approximately 250 days old.
If I remember correctly, Durk Pearson was taking high doses of pantothenic acid.
Looking at the historical development of mouse lifespan studies, we find that the late 70s and early 80s saw a marked improvement in lifespan (Fig. 6A). This is more likely due to improved husbandry rather than a shift towards the use of longer-lived strains since the same trend was observed when we limited our analysis to the popular C57BL/6 strain only (Fig. 6B). After this period of marked improvement, lifespan plateaued around 800 days. This increase in lifespan is consistent with a convergence towards a strain-specific optimum. However, we suggest that further improvements in husbandry and mouse lifespan would enable identification of lifespan-extending compounds and interventions with higher confidence and fewer false-positives.
In the late 50s (time of calcium pantothenate study), lab mice lifespan was in the high 580s, 600.
Regularly eating golden oyster mushrooms protected heart health and promoted longevity in mice.
Golden oyster mushrooms (Pleurotus citrinopileatus) – fungi native to parts of Russia, China and Japan – are one of the richest natural sources of an antioxidant called ergothioneine. Studies in humans have shown the compound is associated with a lower risk of heart disease and premature death. These findings rely on observational data, however, so it isn’t clear whether ergothioneine is driving the health benefits.
After a year, the mice had significantly better heart function than those that weren’t fed the mushrooms. For instance, their hearts pumped about 20 per cent more blood, on average, to the body with each contraction. The mice could also run faster and further, and they had lower levels of genetic markers associated with heart failure.
These metrics usually worsen with age, suggesting that golden oyster mushrooms may protect against age-related declines in cardiovascular health. Further analysis showed the fungus probably does so by shielding cells in the heart and blood vessels from damaging inflammatory molecules known as reactive oxygen species.
The serving size was also enormous, equivalent to an 80-kilogram person eating 720 grams of dried mushrooms a day.
Yeah, I’ve been taking 7.2 grams per day of dried golden oyster for months. That’s one scoop and it’s quite a bit. I can’t even imagine trying to eat 100 scoops. It would be a full time job.
Also, this is a good call but my efforts look like they were wasted on this one. I spent most of a day plugging logs with grains spawn because nobody carries plug spawn. Grain spawn doesnt’ work for several reasons and I have proven it once again. It’s a much better source than golden oyster and should like the climate here better, but I’m still not getting plugs.
Actually now that I look at it, if I could get cornucopiae to grow, thats a factor of almost 10 and if we divide by 12, then my 7.2 grams/day would almost do it.
Yeah, it seems every time I look at a study of components in mushrooms the spread is huge. It depends on conditions when and where it’s grown. I should bother the local labs here and ask what it would cost to test mine if I get a big flush next year, or I could check the powder in my freezer. Maybe I’m just eating dust.
Ergothioneine improves healthspan of aged animals by enhancing cGPDH activity through CSE-dependent persulfidation
Highlights
• Ergothioneine enhances lifespan and healthspan in aged C. elegans and rats
• These effects are dependent on CSE-catalyzed H2S production and persulfidation
• Ergothioneine boosts NAD+ levels
• Ergothioneine-induced persulfidation of cGPDH enhances its NAD+ formation
Summary
Ergothioneine (ET), a dietary thione/thiol, is receiving growing attention for its possible benefits in healthy aging and metabolic resilience. Our study investigates ET’s effects on healthspan in aged animals, revealing lifespan extension and enhanced mobility in Caenorhabditis elegans, accompanied by improved stress resistance and reduced age-associated biomarkers. In aged rats, ET administration enhances exercise endurance, muscle mass, and vascularization, concomitant with higher NAD+ levels in muscle. Mechanistically, ET acts as an alternative substrate for cystathionine gamma-lyase (CSE), stimulating H2S production, which increases protein persulfidation of more than 300 protein targets. Among these, protein-persulfidation-driven activation of cytosolic glycerol-3-phosphate dehydrogenase (cGPDH) primarily contributes to the ET-induced NAD+ increase. ET’s effects are abolished in models lacking CSE or cGPDH, highlighting the essential role of H2S signaling and protein persulfidation. These findings elucidate ET’s multifaceted actions and provide insights into its therapeutic potential for combating age-related muscle decline and metabolic perturbations.
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