Intramuscular (IM) + Intranasal (IN) Rapamycin - A new paradigm for human longevity translation

#165

I’ve used water-based suspensions for some obscure tryptamine and arycyclohexylamine salts in the past, and the bioavailability didn’t seem to be impacted. Ofc rapa is a larger molecule that doesn’t have overt psychoactive effects, so it’s harder to say whether it was actually absorbed.

#166

Title:

Subcutaneous Rapamycin Injection Protocol — 10 mg Weekly Dose, 0.5 ml Volume, DMSO/PEG-400/PBS Vehicle (No Oil)

Hello everyone,

I wanted to share a detailed and practical protocol for subcutaneous rapamycin injections that I have developed, based on discussions here and available literature. The goal was to create a sterile, convenient, and bioavailable preparation without the use of oil vehicles.

Protocol summary:

  • Dose: 10 mg rapamycin per injection
  • Frequency: once weekly
  • Injection volume: 0.5 ml per dose (small volume for comfort)
  • Vehicle composition: 20% DMSO, 40% PEG-400, 40% sterile PBS (pH 7.4)
  • Total preparation: 100 mg rapamycin dissolved in 5 ml vehicle, divided into 10 × 0.5 ml sterile syringes
  • Storage: syringes frozen at -20°C, thawed immediately before use, no refreezing
  • Administration: subcutaneous injection (abdomen or thigh), injection done slowly to minimize discomfort

Preparation steps:

  1. In a sterile environment, dissolve 100 mg rapamycin in 1 ml DMSO until fully dissolved.
  2. Add 2 ml PEG-400 and mix thoroughly until clear.
  3. Add 2 ml sterile PBS (pH 7.4) and mix gently.
  4. Using a 0.22 µm sterile syringe filter, filter the solution directly into 10 sterile insulin syringes (0.5 ml per syringe).
  5. Cap syringes with sterile caps or needle covers and freeze immediately at -20°C.

Notes:

  • This vehicle composition balances solubility and tissue tolerance, avoiding the complexity and sterility challenges of oil-based depots.
  • The protocol aims for practical weekly dosing with acceptable bioavailability and minimal irritation.
  • The volume of 0.5 ml per injection improves patient comfort for subcutaneous delivery.
  • Freezing aliquots individually maintains sterility and stability over 10 weeks.
  • Slow injection technique recommended.

If anyone has experience with alternative vehicles, depot methods, or different dosing strategies, I’d love to hear your feedback.

Thanks for reading!

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