Interesting review of age related biomarkers

Interest review posted on twitter by Mike Lustgarten…I haven’t finished studying but I see a large emphasis on declining NO from falling Arginine with aging. I look forward to feedback from this group.

Review of Age Related Biomarkers


Yes, Michael Lustgarten PhD referenced an article today on Twitter. I must admit that most of the article is over my head. One thing that resonated with me is the age-related inflammation aspect.

“Low-grade chronic inflammation is a feature of aging, and is generally interpreted as a reflection of accumulation of micro injuries and continuous repair (Walker et al., 2022).”

“Regardless of the precipitating pathways, there is evidence that inflammation contributes to the development of many age-related diseases, such as cardiovascular disease, osteoporosis, cognitive impairment, dementia, sarcopenia, and frailty”

I have believed this for many years and I stopped focusing on anti-oxidants and started focusing on anti-inflammatories.

My favorite anti-inflammatory is Boswellia Serrata (Frankincense). I think this supplement will be more recognized in the future as one of the top OTC supplements that you can take for age-related inflammation.

I really would be interested in finding out the favorite anti-inflammatory supplement any of the forum members are using.

“Boswellic acids have anti-inflammatory and antiarthritic effects, so that they are finding pharmacological use in both East and West. These compounds seem to work by preventing the body from making pro-inflammatory compounds, whilst they also exert antitumor effects in colorectal cancer cells.”,in%20both%20East%20and%20West.


Vincent E. Giuliano, Ph.D. is big on Boswellia. He includes it in his 4 Herb Synergy product that he says he has been taking for a long time. He swears it is the key to his longevity (93yo).


This is my go to anti-inflammatory too. I stopped taking it for a while and at my last blood panel my hsCRP was up around 4.0. Double the previous value. Of course, as John has noted hsCRP can go sky high during/after an infection but in my case I wasn’t aware of an infection. So I am back on the boswellia in the form of ApresFlex (5-lox inhibitor from Life Extension).

Also taking a ginger extract which has some standardised amount of gingerols and occasionally a Garlic extract with a standardised amount allicin but that often gives me heartburn. Both have evidence that they act on NF-κB. Lets see what my next blood panel says.


Yes. I’ve been reading Vince for many years and he does emphasize chronic inflammation and taking anti inflammatory supplements.

He’s very smart and probably knows more about the cellular processes involved in aging than almost anyone.

It’s interesting that he’s not on rapamycin.


Vince said he was on rapamycin in the past but stopped due to recurring infections.


Very interesting. I wasn’t aware that Vince ever tried it. He was susceptible to the innate immune suppressant aspect of the drug .
It’s very possible that rapamycin has significantly different effects in different individuals, which makes it sort of a crap shoot without a great deal of data.


@desertshores I have used curcumin for a long time and tried different formulations. The one that suits my needs the best is Life extension’s Super Bio-Curcumin 400 mg - BCM-95® Bio-Curcumin® extract. Sometimes I combine super Bio Curcumin with thorne’s quercetin phytosome 250 mg and green tea extract. I take the green tea extract and quercetin about 1-2 hour before the curcumin. .This combination give me a profound cognitive boost of energy and almost an euforic mental state.

My second best curcumin product is Longvida optimized Curcumin 500mg.

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@Joseph_Lavelle There is data that links declining NO to detoriating health. They say that providing L-Arginine is not the best way to facilitate a healthy level of NO. there are candidates like Citruline and taldalafil. One observation is that citruline not only increase NO but also inhibit arginase., which might be a contributing factor to many disease states.

“Diminished bioavailability of nitric oxide (NO), the gaseous signaling molecule involved in the regulation of numerous vital biological functions, contributes to the development and progression of multiple age- and lifestyle-related diseases. While l-arginine is the precursor for the synthesis of NO by endothelial-nitric oxide synthase (eNOS), oral l-arginine supplementation is largely ineffective at increasing NO synthesis and/or bioavailability for a variety of reasons. l-citrulline, found in high concentrations in watermelon, is a neutral alpha-amino acid formed by enzymes in the mitochondria that also serves as a substrate for recycling l-arginine. Unlike l-arginine, l-citrulline is not quantitatively extracted from the gastrointestinal tract (i.e., enterocytes) or liver and its supplementation is therefore more effective at increasing l-arginine levels and NO synthesis. Supplementation with l-citrulline has shown promise as a blood pressure lowering intervention (both resting and stress-induced) in adults with pre-/hypertension, with pre-clinical (animal) evidence for atherogenic-endothelial protection. Preliminary evidence is also available for l-citrulline-induced benefits to muscle and metabolic health (via vascular and non-vascular pathways) in susceptible/older populations.”

"NO is a multifunctional signaling molecule, intricately involved with maintaining a host of physiological processes including, but not limited to, host defense, neuronal communication and the regulation of vascular tone. NO is one of the most important signaling molecules in our body, and loss of NO function is one of the earliest indicators or markers of disease. Clinical studies provide evidence that insufficient NO production is associated with all major cardiovascular risk factors, such as hyperlipidemia, diabetes, hypertension, smoking and severity of atherosclerosis, and also has a profound predictive value for disease progression including cardiovascular and Alzheimers disease. "


While it might be useful for some type of age-related conditions or disorders, the ITP testing suggests its of no help with aging more generally…

We report here on the results of lifelong treatment of mice, beginning at 4 months of age, with each of five agents, that is, green tea extract (GTE), curcumin, oxaloacetic acid, medium-chain triglyceride oil, and resveratrol, on the life span of genetically heterogeneous mice. Each agent was administered beginning at 4 months of age. None of these five agents had a statistically significant effect on life span of male or female mice,



Curcumin wayyyyy overrated. For me it caused severe inflammation as opposed to being anti inflammatory. But 3 spoons ground flax seeds daily were godsend against inflammation. The word has it, flax seeds have HUGE Anti cancerous properties also.

I am a fan of l-citrulline. I used it quite a bit when I took it before heavy workouts. One way I proved it was more effective than arginine is by taking my blood pressure before and after the recommended doses. (I don’t remember what the recommended doses were, I just used the doses recommended by the supplement supplier).
L-Citriline always lowered my blood pressure more than L-arginine. I believe this is an indication that l-citrulline produced more NO. Of course, I am only n=1.


I use it mainly for the cognitive benefits and increased wellbeing. . I speculate, that in my case it brings down systemic inflamation.

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I take both 5mg Cialis and L-Citrulline but I wonder if I even need the citrulline if I am already taking cialis. Plus I eat a cup of watermelon every day.

What dosage do you take?

I take two capsules right after dinner. I am currently using the DoubleWood brand because it has no fillers.


Took it for first time this morning and my bones, joints and muscles loved it, so have to love it also lol. Jokes aside, it did seem to reduce inflammation in my hands and everywhere in the body quite noticeably, so it is a keeper for me. Thanks for the heads up, first time trying it.

Thanks. I’ve now added Boswellia and Citrulline. Now I have to kill off some old supplements to keep reducing my supplement load (a goal).


A cup of watermelon is more like not eating at all lol. for me it’s either 1/2 of watermelon or nothing. hahaa

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Luteolin seems interesting:

I just discovered a reliable source of Luteolin where I am located and I am going to combine it with sulforaphane (Nutrinov Prostaphane):

I have had a continuous bloodshot eye and swollen upper and lower lids for months now. Hoping these combined will help along with hot compress and tea tree oil wipes. Will also monitor my hscrp and post blood test results on my thread here.