An unusual study of fruit flies in that it studied combinations of therapies:
https://www.nature.com/articles/s42003-022-03524-4
Lifespan
The effects of different experimental conditions (maintaining in the dark (DD), low ambient temperature (18 °C), exposure to geroprotectors (3G), dietary restriction (DR)) with potential geroprotective activities and its possible combinations on the lifespan of the w/w control line and long-lived mutants E(z)/w were analyzed.
The strongest increase in longevity was observed in w/w and E(z)/w flies which were kept in a combination of all studied factors
The geroprotectors (3G) were berberine, fucoxanthin, and rapamycin.