Yeah I just searched the landscape and there really isn’t much. It makes me wonder how many humans must have taken this, ever…. Not sure why I volunteered to do it on whim. Have been feeling a bit sluggish lately so any boost in energy will not go unnoticed.
How long will the wormbot test take? I may leave it on a shelf until it comes out.
I am not dealing with ora.
How where you planning on taking?
What dose?
Showing metabocard for Indolepropionamide (HMDB0240640)
So many unanswered questions. If this compound indeed extends lifespan, it may help explain why some individuals live longer.
Gut bacteria convert tryptophan to indolepropionic acid (IPA).
“The gut bacteria that convert tryptophan to indolepropionic acid are Clostridium sporogenes (C. sporogenes).
Indolepropionic acid (IPA) is a precursor to Indolepropionamide (IPAM) in the gut. IPAM is formed through the amidation of IPA, a process carried out by gut bacteria.
Here’s how it works:
IPA Production: Dietary tryptophan is metabolized by gut bacteria like Clostridium sporogenes into Indole and subsequently into IPA.
IPA Amidation: Once IPA is formed, gut bacteria can convert it into IPAM through an amidation reaction, where the carboxyl group (-COOH) of IPA is replaced with an amide group (-CONH2).
In summary: Gut bacteria first produce IPA from tryptophan, and then they can further metabolize IPA into IPAM through an amidation reaction.”
“The pathway for IPA production from tryptophan in C. sporogenes is thought to involve a two-step process:
Tryptophanase (TnaA) first converts tryptophan into indole.
Tryptophan amino transferase (Tam1) then converts indole into IPA.”
C. sporogenes
C. sporogenes is a common gut bacterium."
“It is commonly found in the human gut microbiome, although it might not be present in every individual, or its relative abundance might be lower compared to some other dominant gut bacteria.”
So, how do we increase the amount of C. sporogenes in our gut?
C. sporogenes is known to thrive on amino acids, using a process called the Stickland reaction for energy. Therefore, ensuring adequate intake of dietary protein, which is broken down into amino acids in the gut, may support its growth.
A diet rich in various types of dietary fiber (prebiotics) can promote overall gut health and create an environment conducive to beneficial bacteria, including certain Clostridia.
Sources: Fruits, vegetables, whole grains, legumes. Specific prebiotics like inulin, fructooligosaccharides (FOS), and xylooligosaccharides (XOS) feed beneficial gut bacteria.
“Proteolytic Clostridia are a group of anaerobic bacteria that have the unique ability to grow with amino acids and peptides as their sole energy source. These microbes colonize the gastrointestinal tract of mammals”
So, maybe some people only need to increase or supplement their tryptophan intake to reap the benefits of Indolepropionamide.
As a subnote: I now think that spore-based probiotics are the best probiotic supplements.
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UPDATE: Fundraiser will be up soon, but Ora had accidentally misquoted and the price will be $741.84 instead of $400.
I can still only afford to put $100 towards this at the moment, hopefully we can get it funded soon. I know other people said they would have put some money into this if it wasn’t already fully funded (when we thought it was $400.)
If anyone else is willing to put a bit of money towards this so we can get the study going that would be amazing.
I have just signed the agreement. I will update the post and make another comment here when the fundraiser link is live on the Ora Biomedical website!
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Just curious John are there any species between worms and mice that you think are worth doing lifespan studies on?
I’m thinking if this worm lifespan study is successful and it is the most effective lifespan extension in C Elegans to date, after maybe trying some combination tests and higher dose tests it would be worth figuring out the next higher species to test this on and start a fundraiser for that with the aim of hopefully catching the attention of people like Dr. Aubrey de Grey from LEVF and Dr Richard Miller from ITP and others who are doing mice studies to include this in their testing.
Thanks for the update! 
Have you already considered the dosage for the test? It’s a single-shot study, right? Would it make sense to use 30 µM IPAM—the concentration that showed the highest efficacy in rotifers—to hopefully achieve a significant lifespan extension?
That is exactly what we are doing yes. Additionally since they will be purchasing 5 grams of the substance there should be more for other people to do their own tests with afterwards, either in higher dose or in combinations. If this is true, this means we would do additional tests for the usual $100 price.
Once it is complete, assuming we get positive results, I would like to do 3 additional dose escalation tests 2x, 4x and 8x trying to find the point of diminishing returns. This would highlight the most effective dose, and from there combination studies will be done.
I’m excited, just waiting for Ora to get back to me with the fundraiser link and we can begin 
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Awesome! Maybe you could add the strategy to your original post so people can see it right away—and maybe even join us and contribute. I can’t wait for the worm bot to get up and running! 
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I will edit the original post once the fundraiser link becomes available. I will be sure to include what I just stated in the edit. Great idea katiii.
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Thanks for coordinating this effort, please ping me once the fundraising link is up and running!
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Named after Umbrella Corporation. Well, that might work out, or not. And do you know of the nuances of testing, whether mass spectrometry is appropriate or enough?
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The irony is not lost on me here 
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Great work in finding this compound and driving the research forward! My lab intiative Rapamycin Longevity Lab can help out with a contribution.
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Thank you Krister! I appreciate the support on this project.
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Drosophila can be a good model.
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Can we not just all pay Ora, does it need a fundraising link?
It does need the link unfortunately. They should get back to me soon, this will be the beta test of a new feature on their website. The link will go to a page that has the capability of receiving multiple payments.
Trust me I am eager to get this going as well!
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I can interrogate ChatGPT for a deep dive into nuance but from my understanding, mass spectrometry, the way they use it, is the golden standard.
What reassures me the most is that the founder shows his face on the YT video, gives his name, and interviews the point person at the Arizona lab they subcontract all their Q&A to. The guy looks like an indie death metal band member but is an industrial engineer… so…. Yes, he spoke very minutely over how to conduct and interpret mass spectrometry analysis and it was pretty reassuring. Watch the video I linked to above.