In this research, we found that UA improved cognitive dysfunction and reduced Aβ deposition in APP/PS1 mice. Furthermore, UA activated autophagy and upregulated the levels of autophagy-related proteins in both APP/PS1 mice and Aβ1–42-injured N2a and PC12 cells. At the same time, UA down-regulated the phosphorylation level of PI3K/AKT/mTOR and up-regulated the phosphorylation level of AMPK in APP/PS1 mice and Aβ1–42-injured N2a cells and PC12 cells. In addition, UA down-regulated VDAC1, consistent with the effect of VDAC1 antagonist DIDS (4′-diisothiocyano-2,2′-disulfonic acid stilbene). Importantly, the UA-induced activation of autophagy and modulation of the PI3K and AMPK pathways were reversed by VDAC1 overexpression.
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Given the recent wormbot data, this substance may be good for neuroprotection, but not for general longevity.
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Other labs found positive results on worms so I wouldn’t attach too much importance to one negative result on worms. SGLT2i failed on worms with Ora.
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From ConsumerLab
“In terms of quality, tests in 2025 by the group SuppCo found that only four out of 10 products contained their claimed amounts (or somewhat more of urolithin A: CodeAge Liposomal Urolithin A capsules, Neurogan Health Pro+ Urolithin A, Pure Encapsulations Renual, and Timeline Mitopure Urolithin A. ConsumerLab analyzed the cost to get 500 mg (as listed) of urolithin A from these four products and Neurogan was, by far, the least expensive at just 83 cents (from one 500 mg capsule), while the cost was $3.33 from Codeage (two 250 mg capsules), $3.75 from Timeline (two 250 mg softgels), and $4.87 from Pure Encapsulations (four 125 mg capsules). Timeline and Pure Encapsulations were the only products to list Mitopure (see above) as their source of urolithin A and each included additional ingredients such as resveratrol and CoQ10. Five products were discovered to contain less than 0.1% of their listed urolithin A: Migcopat NAD+ Urolithin A, Pepeior Urolithin A+, Sundhedsliv Urolithin A 1500 mg, Totaria Health Urolithin A NAD+ CoQ10 Resveratrol PQQ, and CystoRebalance Urolithin A. One product, PureHealth Max Urolithin At, contained only 2% of its listed urolithin A. Contamination with heavy metals was found not to be an issue.”
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The supplement game is always riskier than people think, unfortunately.
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FWIW, I was using gethealthspan’s UA, but recently switched to Aeternum for their better price
I read some comments on this forum saying they thought it was a legit brand, however it was only a handful of comments. (If anyone thinks differently, please chime in!)
I bought it during one of their many sales. Note, each serving is 1000mg, and each pill is 500mg, so it’s a better value that it appears at first glance.
also, no fillers.
And, I see no evidence that Timeline contains CoQ10? I am curious because I need to take that and getting two things in one would be appealing.
I’d only use one of the brands ConsumerLab tested and approved if I were to ever buy Urolothin A
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Again: even assuming UA is all that, there are no studies that I am aware of which show what interaction, if any, there is between UA and rapa and/or SGLT2i or other drugs/supplements. Are you willing to take UA without any information regarding such potential interactions? I am not, because I have researched rapa and some of its interactions with some drugs (such as SGLT2i) that I also take, and since I like what I read about these, I don’t want to risk throwing UA into the mix potentially interfereing with them. I will wait for more information before even thinking of getting on UA. YMMV.
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Excellent points, as always. And I always appreciate how exacting you are.
Because, as always, I have zero clue, I did run by the idea of taking it by a smart longevity doc, the one we all know and love, and he felt it was probably a worthwhile thing for me to add, and knows I’m on dapagliflozin and rapa.
I’m definitely not married to it and assumed it was a no harm and might possibly be beneficial proposition.
I’d want to be more convinced of benefits before spending $$$ on Time Line. Having said that, if it also contained the coq10 I need (I’m was shown to be deficient), I’d consider paying for the convenience of taking one thing vs two, especially because they have some new gummies that are supposedly yummy :).
There’s no evidence that Q10 supplementation offers benefit in Q10 deficient people.
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The best evidence for CoQ10 supplementation is in heart failure patients.
But it is true that the blood test isn’t reliable. You need a muscle tissue analysis for that.
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My point regarding deficiency was related to that: Coenzyme Q10 (CoQ10) U-shaped dose-response relation with blood glucose and blood pressure - #41 by adssx
Q10 might have other benefits. However, the paper you cited says: “Patients with heart failure were given coenzyme Q10 at doses ranging from 30 mg to 400 mg per day in the studies included. There was no determination of the minimum and optimal doses for coenzyme Q10 use, and further dose-response analyses will be required in the future.” And we know that Q10 has a weird dose-response: Coenzyme Q10 (CoQ10) U-shaped dose-response relation with blood glucose and blood pressure
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The eminent researcher of mitochondria, Dr. Mark Tarnopolsky, MD, PhD, gave an interesting interview about mitochondria in general and ways to boost them and preserve their health. In passing, he expressed extreme scepticism toward the utility of Urolithin A, and debunks some of the prominent studies of Urolithin A (approx. minute 25). He’s also pretty unenthusiastic about NAD.
Dr Mark Tarnopolsky, MD, PhD | Mitochondrial Mastery: Supplements, Exercise & Longevity Insights (degrees of health)
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Our multi-omic analyses reveal that UA reorganizes ER, mitochondrial and lysosomal networks, linking inter-organellar dynamics to mitochondrial quality control.
In C. elegans, UA induces calcium release from the ER, enhances lysosomal activity, and drives DRP-1/DNM1L/DRP1-mediated mitochondrial fission, culminating in efficient mitophagy.
Similarly, in mammalian cells, UA increases intracellular calcium, enhances mitophagy and mitochondrial metabolism, and mitigates stress-induced senescence in a calcium-dependent manner.
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For anyone interested in Timeline Urolithin A, it is on sale now, 40% off. I just bought 3 on Amazon…
Neurogan price for their Urolithin A product looks tempting but they were unable to provide me a 3rd party COA showing how much Urolthin A was actually in the product. I doubt there is any.
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Thanks Stan!
I was never willing to spend on those, but I’ve been curious about the gummies, so I pulled the trigger and chose the subscription for 46% off!
FYI, I checked and saw they are offering 40% off on their website, but they link Amazon as an option, and that way I could lower the price a touch by choosing subscribe and save. If they didn’t offer that link, I would not have known I could trust the amazon source.
Still most likely overpaying for no reason, but if I can have a yummy gummy, it was worth a gamble. FWIW, I’ve been taking Aeternum brand.
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Can you provide the link to the Timeline product? Thanks.
Either Amazon or Timeline. Try these:
https://www.amazon.com/ Search for Timeline Urolithin A
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ConsumerLab reports that tests done by SuppCo showed that Neurogan, CodeAge, Pure Encapsulations and Timeline contained the claimed amount.
Neurogan being the cheapest.
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I tracked down that report and yes, Neurogan did pass their testing as having the claimed amount of Urolithin A.
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