Correct, the correct dose is .5pg per day.
500mcg is literally a billion times higher
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They do require prescription, but intake exam and prescription is included in the $1800 cost!
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Because it’s not alpha klotho, I have no idea if that is a good value or not. Based on the owners demeanor, I’m guessing it’s not, but I have no way to know for sure because I’ve never seen talk about taking that particular peptide.
Your thoughts?
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Same for me, I had not heard of it until this discussion.
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Roll call:
Headcount on those taking a-klotho here? I’m simply curious. As far as I can tell, it’s about 3 
I recently started taking it and contacted the company about some questions I had regarding shipping and storage. He was impressively responsive and thorough.
With his permission, I’m sharing what I learned here:
Shipping: Even thought they ship it with icepacks, if you are not in a hot climate or ordering in the heat of the summer, it should be fine shipped regular mail and you don’t need to pay extra for expedited shipping.
Storage: If you have undiluted vials, you can store them in the freezer. Avoid freeze thaw cycles, but once is fine and it should be good for a year.
Dilution: I received an unexpected and brilliant tip about this which is what prompted me to post. Once you dilute it, the vial would be good for aprox a month in the fridge, with some slight degradation. The rockstar tip he shared was to fill all your syringes at once, and then store those syringes in the freezer. Yes, I said the freezer! You then pull out a syringe daily! Now your dilution will last for several months!
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Some peptides are OK to freeze once reconstituted. Some are not. Good to hear Klotho fragments are.
The human Klotho molecule is a large protein (1012 amino’s) and the more commonly available “Klotho” peptides are fragments of that molecule. This is a common process.
Another example would be TB500, there are 3 versions, the common one is Frag 1-4, the next common version is Frag 17-23 while the full version of thymosin β4 is 43 amino’s.
Here are 2 klotho derived peptides.
https://www.tocris.com/products/klotho-derived-peptide-6_7847
https://www.tocris.com/products/klotho-derived-peptide-1_7830
Apparently there are 18 Klotho derived peptides. which begs the question, which one are you getting? Seems like Klotho 6 has a bit more clinical evaluation.
According to research, there are currently 18 identified Klotho-derived peptides which are typically designed by dividing the KL1 domain of the human Klotho protein into overlapping peptides, each roughly 30 amino acids long, allowing for comprehensive study of different peptide sequences within the Klotho protein
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Beth, Alpha Klotho has been on my investigation list for a long time, but the dosing protocol found on FaceBook’s Klotho AntiAging group seems nebulously derived. I believe the reasoning behind their 0.5 picogram daily dose was explained and of course you don’t want to overdose, but it’s such a tiny dose compared to existing levels in the body.
On their FaceBook page the Kloth AntiAging group has a reference to dosing (and a link) by the Italian Cyclist Marcello Bergamo using a dose of ~5pg/week, but trying to find further information about Bergamo and Klotho other than that link has failed me so far. So, is that page legitimate or just a low-key promotion to buy Klotho from a site link on that page. And, there is also some reference to Steve Perry about dosing. But, again, it’s nebulous though there was some reference to “trial and error” which may be a very valid point. I am just a very skeptical person.
Using ChatGPT I received speculative HED Dosing (Human Equivalent Dosing) that I will not list here because the data kept changing when I worded the question differently. However, the information was in the ug (microgram) area instead of the pg (picogram) area.
So, if you have time please tell about your dosing protocol and any reasoning behind it.
Thanks for your time.
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Hi Jay! I’ll be happy to share my dosage with you.
I titrated up and am now taking 1mcg per day.
You’ll see that Grant shared his dose in this post, here: New Attia podcast on Klotho - #75 by DrFraser
Sorry that I can’t be helpful in explaining any of the reasoning behind this protocol. I’m a patient of his and trust him, so I happily follow what he is doing for himself If he changes what he is doing, I will follow in lockstep.
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Beth, 1 mcg is more like it and titrating up also makes sense. I assume you’re using the Celia Klotho FG product. I wonder if it’s a peptide or pure alpha Klotho? I’ll ask Dr Fraser. Thanks.
Actually, I’m not using Celia and wouldn’t. I’m using the a-klotho from Bucky Labs. Grant even shared his discount code in that same thread. I think it was Grant!
The Celia is not alpha klotho. After the owner of Celia chimed in on Keith’s post in the FB group, Keith then posted exactly what it is (I can’t remember offhand, otherwise I’d share that here),
Based on her demeanor, I would never feel comfortable buying anything from her. Just one person’s opinion
@Jay I just tagged you on that FB post (her comments have since been deleted)
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As we are getting close to the end of the year, wanted to check if you have any updates to share on this front. Klotho seems primed for entering mainstream longevity discussions so curious as to your experience and effects, or anecdotal feedback. Are you still using Bucky’s?
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Hi @One,
I wish I had something useful to share with you.
I used it for 2-3 months, but I stopped for a couple of reasons.
The doc who was advising me no longer felt that affordable doses were enough to be effective.
I still thought, well, as with many other things, perhaps some is better than nothing, but amongst all of the smart and proactive people here, almost no one uses it. I then asked around in the facebook Klotho group, and almost no one there uses it there either.
With all of that combined, I stopped buying it in fear of it being a waste of money.
It still makes sense to me that a little is better than nothing, but if that were true, I’d think more people here might be using it, so I’m just assuming I’m wrong. I do wonder if more people would use it in small doses if it were more affordable, or if everyone simply thinks it’s a waste of time.
I only purchased it from Buckys. I never found a source to buy it from directly from a vendor in China, where is it no doubt made.
Thanks for sharing.
Some of these, like Klotho, rely so much on hypothetical benefits that I can see that.
There does seem to be a wave of podcasts and email newsletters starting to hype it up again (from “Hunter Williams” at one end of the spectrum to Dr. David Barzilai (@aging doc on X) at the other end) which makes me think that either they have figured out a source to sell more of it or there is some study/publication about to come out on Klotho.
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I believe that the benefits of elevated Klotho are real.
The problem seems to be that exogenous (injected) klotho is so rapidly cleared from the body that it is doubtful it has time to initiate changes. You would need to inject large amounts every day.
The solution seems to be either to work on boosting your own production of klotho, or use a genetic modification to increase expression of klotho. I don’t know of any lab or company offering the latter.
I have Dr Fraser to thank for sharing his pioneering work in klotho.
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Liz Parrish and one of her partners have done this and I think they offer it.
Liz Parrish klotho genetic mod.pdf (280.7 KB)
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Actually the short half life of Klotho is immaterial. It is the PF4 activation that occurs immediately after klotho administration that is important. (For cognitive effects) unknown for whether klotho half life is important for the overall reduction in MACE
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I think the main reasons many people don’t use Klotho, aside from the fact that it isn’t so well known, is the fact that it’s hard to get hold of it and that it requires injection. Only a very small minority of people ever use things that aren’t approved as supplements or drugs or require injections.
Personally I’ve been aware of klotho being potentially beneficial for aging since long before people started experimented with it. My cautious approach has been to simply try indirectly supporting endogenous Klotho production.
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Jay Campbell is selling Alpha Klotho with modifications to extend half life. I doubt it is real or safe and I regard Jay as one of the worst humans in the peptide space. But here is the link for the curious. I would love to know if it can get tested and if anyone can find an Assay for testing it to confirm it is working.
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That’s good feedback, thanks.
I’d normally never jump on anything almost no one is doing, but I felt comfortable trying klotho simply I was advised by a smart doc and Peter Attia and Tim Ferris have said they are about to be guinea pigs in a trial (which to me says they already take it but who knows)
I am curious about so many peptides, but I have only experimented with NAD, glp1, and klotho. I am ordering SS-31 now (felt comfortable after rapadmin met the researcher), and I might add TA-1 to my order to have on hand incase I get sick (really, adding TA-1 it all about getting free shipping because I’m a sucker for that, so I’m still googling to see if there are any potential downsides).
@touringsedan there is a klotho test one can take. If I recall, it might be $300 or so? (Grain of salt on that $) . I definitely don’t have the stomach to test out a new form on myself.
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Thank you Beth for your insight. I too have been on Klotho once a day for a couple of years. Recently Ive really started questioning why I’m doing it! So expensive with untestable results. I am also ordering SS-31. I look forward to hearing about how it works out for us older folks.
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