Question about fasting protocols

Hi…sorry it has taken me so long to respond. Your question addresses one of the many things about rapamycin that have not been adequately tested in humans. I regularly combine intermittent fasting with rapamycin. IN Blagosklonny’s 2019 article titled Fasting and rapamycin: diabetes versus benevolent glucose intolerance…he states " asting and rapamycin: diabetes versus benevolent glucose intolerance."

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4x a year I fast for 96 hours, just electrolytes and water intake. I found an interesting report that adequate intake of spermidine may be required to achieve highest levels of autophagy under those conditions.