I would like to start using Thymosin Alpha 1 and noticed that at least one website claims Thymosin Alpha 1 requires PBS buffer vs the usual bacteriostatic water used for most peptides. Supposedly Thymosin Alpha 1 is acidic and requires an alkaline buffer (PBS is a standard 7.4 PH alkaline buffered 0.9% saline solution stocked in many bio labs). It is not clear if the only benefit of PBS buffer is to prevent the stinging reaction from injecting an acidic solution under the skin, or if it actually is necessary to avoid damaging the Thymosin Alpha 1 peptide.
I can only find it sold as 500ml sterile bottles around $25 on amazon (sterile PBS) and since these are NOT bacteriostatic they would have to be discarded after a single use (of reconstituting a vial of Thymosin Alpha 1). On the other hand these don’t cost a lot more than the 30ml bottles of bacteriostatic water.
Been using TA-1 for about a month, every day. 1.4mg per dose in a 20 unit injection. This is a 10mg vial with 1.4mL bac water = 7 doses. Using standard bacteriostatic water with no issues. 5 of us, no issues.
There are some very specific things to know about TA-1. Once reconstituted is must be used in 5 to 7 days.
As a powder, it is shelf stable at room temp for 3 weeks.
As a powder it’s stable in a refrigerator for 5 to 6 months
As a powder it’s stable in a freezer at -21C for up to 12 months.
This is a very well studied peptide and is an approved drug in over 40 countries. Lots of good data on how it works and why it’s not stable once reconstituted. No mysteries with this one.
We do use it in combination with LL 37 - I put both peps in 1 vial, so we only take one shot
Looks like a breakthrough in understanding how EBV hides and an opportunity to solve this one
Despite its range of disease associations,
Epstein-Barr virus (EBV) treatments or
vaccines are lacking. Joyce et al.
determined EBV gp350 structures
complexed with either its human
receptor, CR2, or virus-neutralizing
antibodies (nAbs), thus illustrating
structural convergence of viral receptor
and nAbs and offering insights for
vaccines and therapeutics for EBV
I’d go out on a limb and say no. This recent understanding of how EBV hides may lead to a solution.
But as a hopeful kind of person, using TA-1 and LL 37 judiciously, plus our modified TRIIM protocol, I’m trying to provide my system with the tools it needs to be a more effective pathogen killing machine. Can I measure this aspect of it? nope. Have I seen results from these 2 peptides with other pathogens, yes I have.
I have wonderful results fom Cerebrolysin after ten 5 ml IM injections over a 20-day course, starting after around injection nr 6. Vindicating all those anecdotal reports claiming great results, although I will add that I can’t be 100 % certain that placebo hasn’t played a role in my case.
Can this be sustained and how, should I wait six months before a new round? The anecdotal reports are all over the place. Will decide on whether to use my remaining five 5 ml bottles now or later.
If your condition has advanced to osteoarthritis, you might consider intra-articular injections of the peptide AOD-9064 with hyaluronic acid, which can help to regenerate cartilage. Finding a practitioner in your area may be difficult.
AOD 9604 + HA DESCRIPTION:
AOD9604 is a GH fragment which comprised the last
16 amino acids of the larger growth hormone molecule.
Although originally studied for fat loss, further studies have
transitioned it’s application for regenerative medicine. In
combination with hyaluronic acid (HA), it is now being
used to help regenerate hyaline cartilage and is showing
strong efficacy in the treatment of osteoarthritis. The
combination acts to enhance the differentiation of adipose
mesenchymal stem cells into bone, promote proteoglycan
and collagen production in chondrocytes, and promote
differentiation of myoblasts into C2C12 cells; all of which
are essential for bone, cartilage, and muscle repair. These
studies indicate that it has stronger therapeutic benefits
compared to Bone Marrow Aspirate Concentrate (BMAC)
and Platelet Rich Plasma (PRP) therapy, which have also
been emerging as candidates for osteoarthritis medications.
AOD9604 + HA has proceeded to human WOMAC trials
which allow the combination to be investigated for on an
osteoarthritis index which considers pain, stiffness, and
functionality on a variety of scores.
That reference guide is really helpful thanks @AgentSmith
I’ve gone pretty deep down the peptide rabbit hole and once you’re using a few simultaneously it helps to track their dosages. There’s a number of peptide calculators, I use peptidecalc.io on the app store and it’s been great. It’s the only one I’ve found that will do picograms say for klotho and IU for HGH for example.
Oh. That’s me trying to ramp up. I have some auto immune issues and I’m apparently pretty sensitive to TA-1. It gave me insomnia at higher doses so I’m taking it easy this time.
My PP - personal physician/practioner wants me as high as possible in accepted normal range without going over… areas like B-12… D… Testosterone. So high normal. But, not flagged on test as too high.
To get my D levels up started getting natural sun 20 minutes in swimsuit every 4 -5 days. (after avoiding sun for almost 2 years) and taking higher D3 supplements. Checking levels every 4 months and tweaking.
Probably some here don’t know that you inject weekly, and usually doctors will have people test right before their injection to get a trough, but maybe your 1400 is closer to the peak? And I don’t remember if you get LC/MS or the immunoassay as the latter can be off by quite a bit. And maybe your SHBG is high so free T is in range. All this is to say that your level of 1400 might be less shocking to some with more details. Or perhaps that is your trough and your free T is actually supraphysiological, ha.
@Agetron Just curious, but have you thought about microdosing to reduce peaks and troughs? Obviously, what you’re doing now works, but wanted to throw that out there. I microdose every other day and it works well for me. I don’t seem to have any ups and downs in energy or mood.
