I wouldn’t put a lot of value in a study with such short-lived controls: The Impact of Short-lived controls on the Interpretation of Lifespan Experiments and Progress in Geroscience
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Below is the Kaeberlein paper establishing the 900 day rule.
https://www.biorxiv.org/content/10.1101/2023.10.08.561459v1.full Control lifespans over the years – a need for further improvement
Looking at the historical development of mouse lifespan studies, we find that the late 70s and early 80s saw a marked improvement in lifespan (Fig. 6A). This is more likely due to improved husbandry rather than a shift towards the use of longer-lived strains since the same trend was observed when we limited our analysis to the popular C57BL/6 strain only (Fig. 6B). After this period of marked improvement, lifespan plateaued around 800 days. This increase in lifespan is consistent with a convergence towards a strain-specific optimum. However, we suggest that further improvements in husbandry and mouse lifespan would enable identification of lifespan-extending compounds and interventions with higher confidence and fewer false-positives.
In the late 50s (time of calcium pantothenate study), lab mice lifespan was in the high 580s, 600.
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https://www.amazon.com/L-Ergothioneine-25mg-Vegetarian-Antioxidant-Plant-Based/dp/B0D6J1FJV8
How about this product? The price is cheap.
I’m not familiar with the brand. Being cheap raises questions of quality as the best ingredients that we want are not that cheap.
That being said, I do take Nutricost’s mushroom supplement for ergothioneine. I have experience with the brand and it is cheap too.
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Regularly eating golden oyster mushrooms protected heart health and promoted longevity in mice.
Golden oyster mushrooms (Pleurotus citrinopileatus) – fungi native to parts of Russia, China and Japan – are one of the richest natural sources of an antioxidant called ergothioneine. Studies in humans have shown the compound is associated with a lower risk of heart disease and premature death. These findings rely on observational data, however, so it isn’t clear whether ergothioneine is driving the health benefits.
After a year, the mice had significantly better heart function than those that weren’t fed the mushrooms. For instance, their hearts pumped about 20 per cent more blood, on average, to the body with each contraction. The mice could also run faster and further, and they had lower levels of genetic markers associated with heart failure.
These metrics usually worsen with age, suggesting that golden oyster mushrooms may protect against age-related declines in cardiovascular health. Further analysis showed the fungus probably does so by shielding cells in the heart and blood vessels from damaging inflammatory molecules known as reactive oxygen species.
The serving size was also enormous, equivalent to an 80-kilogram person eating 720 grams of dried mushrooms a day.
Read the Full article: Golden oyster mushrooms may stave off signs of ageing (New Scientist)
Research Paper (open access):
Long-term intake of Tamogi-take mushroom (Pleurotus cornucopiae) mitigates age-related cardiovascular dysfunction and extends healthy life expectancy
https://www.nature.com/articles/s41514-024-00191-z
npj aging DOI: 10.1038/s41514-024-00191-z
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The mice ate about 9 grams of powdered mushrooms per kilogram of body weight daily.
They seem to have simply multiplied 9 grams by 80 = 720 grams.
Shouldn’t the mouse dose be divided by 12.3?
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Yeah, I’ve been taking 7.2 grams per day of dried golden oyster for months. That’s one scoop and it’s quite a bit. I can’t even imagine trying to eat 100 scoops. It would be a full time job.
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Also, this is a good call but my efforts look like they were wasted on this one. I spent most of a day plugging logs with grains spawn because nobody carries plug spawn. Grain spawn doesnt’ work for several reasons and I have proven it once again. It’s a much better source than golden oyster and should like the climate here better, but I’m still not getting plugs.
Pleurotus cornucopiae 2082.00
Pleurotus eryngii 317.00
Actually now that I look at it, if I could get cornucopiae to grow, thats a factor of almost 10 and if we divide by 12, then my 7.2 grams/day would almost do it.
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I take pleurotus ostreatus. Can’t find reasonably priced citrinopileatus nor cornucopiae. On the net, they are offered in small quantities.
Searching indicates that ostreatus’ ergo content is reasonably high.
| A. bisporus | P. ostreatus | P. citrinopileatus | |
|---|---|---|---|
| UV–Vis | 7100 (±300) c | 9200 (± 800) b | 8300 (±1100) b |
| LC–MS | 521.2 (±14.7) d | 607.3 (±11.2) c | 822.1 (±20.6) b |
| Method | Lovastatin Content (mg kg−1 Dry Sample) a | ||
| UV–Vis | 1050 (±80) b | 930 (±100) b | 840 (±250) b |
| LC-MS | 1.39 (±0.014) b | 1.11 (±0.042) c | 0.158 (±0.005) d |
There is a bonus small dose of lovastatin when ingesting mushrooms.
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Yeah, it seems every time I look at a study of components in mushrooms the spread is huge. It depends on conditions when and where it’s grown. I should bother the local labs here and ask what it would cost to test mine if I get a big flush next year, or I could check the powder in my freezer. Maybe I’m just eating dust.
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In worms… and rats.
Ergothioneine improves healthspan of aged animals by enhancing cGPDH activity through CSE-dependent persulfidation
Highlights
• Ergothioneine enhances lifespan and healthspan in aged C. elegans and rats
• These effects are dependent on CSE-catalyzed H2S production and persulfidation
• Ergothioneine boosts NAD+ levels
• Ergothioneine-induced persulfidation of cGPDH enhances its NAD+ formation
Summary
Ergothioneine (ET), a dietary thione/thiol, is receiving growing attention for its possible benefits in healthy aging and metabolic resilience. Our study investigates ET’s effects on healthspan in aged animals, revealing lifespan extension and enhanced mobility in Caenorhabditis elegans, accompanied by improved stress resistance and reduced age-associated biomarkers. In aged rats, ET administration enhances exercise endurance, muscle mass, and vascularization, concomitant with higher NAD+ levels in muscle. Mechanistically, ET acts as an alternative substrate for cystathionine gamma-lyase (CSE), stimulating H2S production, which increases protein persulfidation of more than 300 protein targets. Among these, protein-persulfidation-driven activation of cytosolic glycerol-3-phosphate dehydrogenase (cGPDH) primarily contributes to the ET-induced NAD+ increase. ET’s effects are abolished in models lacking CSE or cGPDH, highlighting the essential role of H2S signaling and protein persulfidation. These findings elucidate ET’s multifaceted actions and provide insights into its therapeutic potential for combating age-related muscle decline and metabolic perturbations.
Open Access Paper:
https://www.cell.com/cell-metabolism/fulltext/S1550-4131(24)00490-X
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interesting study on lkm512 Longevity in Mice Is Promoted by Probiotic-Induced Suppression of Colonic Senescence Dependent on Upregulation of Gut Bacterial Polyamine Production - PMC
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Discussed below, in which thread you participated.
There are links to Amazon available products.
Looks like there is an American brand of yogurt that has BB-12.
https://www.amazon.com/Stonyfield-Organic-Whole-Plain-Yogurt/dp/B00108EPCU
Product Description
Stonyfield Organic is making it easier and more delicious to up your probiotic intake – after all, having the right balance of bacteria in your gut is vital to maintaining good health. Treat yourself to a whole milk yogurt with billions of probiotics in each serving, and live and active cultures including S. thermophilus, L. bulgaricus, Bifidobacterium BB-12®, L. acidophilus, L. paracasei and L. rhamnosus. At Stonyfield, we use 100% organic milk from pasture-raised cows and a variety of flavors made with simple ingredients to create a tantalizing snack for adults and kids on the go. Our yogurt is made without the use of toxic persistent pesticides, antibiotics, artificial growth hormones, or genetically modified organisms (GMOs), protecting you for the long haul and contributing to a healthy and sustainable world. With high-quality ingredients and no added nonsense, Stonyfield Organic Yogurt is #goodonpurpose!
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A great talk from Singapore (NUS) about Ergothioneine.
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@adssx they spend more than half the video on Ergo and show very promising things about Parkinson’s. High levels seem to help quite a bit. Long video.
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27:58 shows doubling (almost) of ERG after three months. Supplementation was 25 per elderly patient. (Study had 18 elderly patients as subjects 28:02
28:16 “And in fact, I must apologize, that is a typo. It should be just 25 mg.”
Dosage is doable with oyster mushrooms, at about one tablespoon (mushroom powder) a day. Even shiitake (lentinula edodes) can provide the dosage at two tablespoons powder per day.
The biology of ergothioneine, an antioxidant nutraceutical - PMC)%2C%20stand%20out(6%2C
Those with the highest amounts include oyster mushrooms (Pleurotus spp., up to 4 mg/g DM)(306), the golden oyster Pleurotus citrinopileatus with 10·65 mg/g DM(307,308), and shiitake (Lentinula edodes , about 1 mg/g DM), while of those more common outside Asia, porcini or ceps (Boletus edulis , > 7 mg/g DM), stand out.
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I weighed my little scooper full of golden oyster (home grown and sun dried) I take every morning. 7.5 grams dried powder. 7.5*10.65= almost 80mg. I’m getting plenty. I think there’s 2 years worth in the freezer, which I’ll need since I tried something that didn’t work last year. Mushroom business is hit and miss like so many other things.
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I just cover all the major mushrooms with this.
It’s $0.12 each on Amazon. Not bad!
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Yes 25 mg and it was only only taken three times a week.
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We found that mice fed a HF-SHM (high fact-shiitake) diet had ∼86% smaller aortic lesion area than mice in both HF-C (P < 0.01) and MIX-C (P < 0.01) groups and also expressed 31-48% lower vascular cell adhesion molecule-1 levels (P < 0.05) than all other groups. Similarly, HF-PBM- (high fat-portobello) fed mice displayed a 70% reduction in aortic lesion area in the tricuspid valve only (P < 0.05). Both mushroom-fed groups had lower weight gain and fat mass (P < 0.05) than the control groups.
Full article below.
https://www.sciencedirect.com/science/article/pii/S0022316622166897?via%3Dihub
Human equivalent dosage is huge.
According to Wu et al. (12), 10% wt:wt mushroom powder in mice will translate to ∼11 g fresh mushroom/kg body weight, which is ∼750 g mushroom/d (10 servings/d) for a person with an average weight of 65–70 kg.
Assuming the powder is one tenth of fresh mushrooms, that is still 75g (five tablespoons) of powder per day. If the effects scale down linearly, then two tablespoons of powder would produce 40% of the benefit. That is a big if.
The full study has a pic of the lesion area.
LF-C low fat control
HF-C high fat control
HF-PBM high fat portobello
HF-SHM high fat shiitake
MIX-C mixed control
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