How long does it take for NAD+ levels to build up after NAD+ supplementation?

#1

(I once got tested after several days of extra-heavy supplementation and my NAD+ levels were still low)

#2

FWIW Sinclair says here that 1g NMN doubles NAD after 2 weeks:

Note he dedicates over 20 minutes to NAD precursors and only 2 to Rapamycin :roll_eyes:

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#3

What about Niagen vs NMN?

#4

At least he admitted that rapamycin almost certainly will prove to extend lifespan in humansā€¦

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#5

Thats more of a religious debate at this stage of the research.

Neither compound has shown life extension capability in mice (repeated in multiple labs - NR failed in the ITP studies, though david Sinclair does claim it with NMN in his latest podcast.

#6

It doensā€™t increase LS, but thereā€™s evidence for healthspan and for energy levels, and I have chronic fatigue issues so Iā€™d like to know anyways

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#7

The problem with measuring NAD levels is where? Apparently you can increase plasma levels with a precursor like NR or NMN but there is no evidence that the increase also happens in the cells of the body.
Another approach i have been following is reducing CD38, which is blamed for destroying NAD+.

To do that you need to fin a source of Apigenin

Iā€™ve been using donotage.com in New Zealand

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#8

I do feel a boost after taking 1g of NMN.

For now I will keep taking it, even though Iā€™m acutely aware of the discussion about its effects or lack thereof.

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#9

NMN vs Nicotinic Acid: Which Is Better For Increasing NAD?

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#10

This was a great video. I already drank the cool-aid on NR in my past (an unpleasant but necessary step in my development as a critical thinker on longevity). I now take (in rotation) niacin and nicotinamide for NAD, AGEs, and more.

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#11

New research from the Treebak Group from the NNF Center for Basic Metabolic Research at the University of Copenhagen suggests that it may be a waste of money. In a study publishedin the journal Cell Metabolism, scientists found that reducing skeletal muscle NAD+ levels in mice by 85% did not accelerate aging or impair whole-body metabolism.

ā€œOur results suggest that skeletal muscle can tolerate substantial NAD depletion without loss of function or accelerated aging,ā€ says Associate Professor Jonas Treebak, corresponding author of the study. ā€œThis challenges the prevailing view that NAD+ decline is a primary driver of muscle aging and frailty.ā€

The source Paper:

More information: Sabina Chubanava et al, NAD depletion in skeletal muscle does not compromise muscle function or accelerate aging, Cell Metabolism (2025). DOI: 10.1016/j.cmet.2025.04.002

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#12

Nice article.
Is there any vitamin or mineral that can (or shown to) increase lifespan or none?
I did not see any in the ITP. Maybe thereā€™s been other testing done on them but not sure.

However, with some of the various age tests out on the market (rejuvenation olympics etc), it seems certain products/supplements can lower(slow) the speed the aging.
Such as Novos products, Bryan Jā€™s stack, Dave Pā€™s stack etc.
Not sure how legit all the testing is.
Not sure what product(s) are improving those tests the most. Any idea which products work best here?