How do you find/sequence de novo genetic variants not in SNPedia or with a rsid?

#1

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100x might be more helpful than 30x. PacBio?

#2

30× singleton
okay for normal variant cataloging
weak for “unique causal variant” hunting

100× singleton
much better for high-confidence private variants
still cannot establish de novo

100× singleton + long-read
better for SVs, repeats, phasing, difficult regions

100× singleton + targeted validation
best for not fooling yourself about top candidates

100× singleton + sibling/relative genotype
useful partial substitute for trio logic