Is it reliable? Responsive to CR?
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[they use the painless tasso, i still dont know if this is evaluated to be legit by 3rd parties
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But even when we focus on klotho and the role it plays in ageing, we rub against a common problem in biomedical research: what weāre using to look for klotho is not all that reliable. Antibodies are commonly used to detect a protein in the lab. The antibody is supposed to recognize a protein of interest (and only that protein). Once it is bound to it, it will be bound to by a second antibody that carries something visible or detectable, like a colour pigment or a fluorescent molecule. The issue is that many commercially available antibodies meant to be specific for one protein are revealed not to be, and tests done using an antibody from company A often do not match the same tests done using a similar antibody from company B, and antibodies sold for klotho detection have not escaped from this problem. Therefore, some of the knowledge weāve acquired about where klotho is in the body and what happens to its levels in this or that condition may not be correct. More rigour is needed to vet the very tools used to study klotho.
That hurdle hasnāt stopped scientists from speculating about and, in some cases, testing actual therapies that involve increasing the levels of klotho in the body, often in laboratory animals. Here too, however, we face challenges. Simply ingesting the protein wouldnāt work, as our digestive enzymes would chop it up into its building blocks, to be used to assemble different proteins in our body. Injecting people with the protein would be of no value in trying to reach the brain, for example, because klotho is so big, it is not thought to be able to move through the blood-brain barrier, a sort of anatomical border control that limits which molecules and microorganisms can enter the brain and potentially harm it. Scientists are thus experimenting with gene therapy and with small molecules that can increase the natural production of klotho protein in the body.
The issue is that many commercially available antibodies meant to be specific for one protein are revealed not to be, and tests done using an antibody from company A often do not match the same tests done using a similar antibody from company B, and antibodies sold for klotho detection have not escaped from this problem. Therefore, some of the knowledge weāve acquired about where klotho is in the body and what happens to its levels in this or that condition may not be correct. More rigour is needed to vet the very tools used to study klotho.
That hurdle hasnāt stopped scientists from speculating about and, in some cases, testing actual therapies that involve increasing the levels of klotho in the body, often in laboratory animals. Here too, however, we face challenges. Simply ingesting the protein wouldnāt work, as our digestive enzymes would chop it up into its building blocks, to be used to assemble different proteins in our body. Injecting people with the protein would be of no value in trying to reach the brain, for example, because klotho is so big, it is not thought to be able to move through the blood-brain barrier, a sort of anatomical border control that limits which molecules and microorganisms can enter the brain and potentially harm it. Scientists are thus experimenting with gene therapy and with small molecules that can increase the natural production of klotho protein in the body.
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I ordered the Klotho test from Longevitylabsolutions.com, but havenāt taken the test yet.
I also ordered a Jinfiniti Aging SOS Advanced panel that includes alpha-Klotho and my results from the blood draw on Jun-17-2024 came back recently and my Klotho number was 0.7ng/ml (Jinfiniti supplies a reference range of 5 - 30 ng/ml for youthful & healthy Klotho levels and anything under 3ng/ml listed as very deficient). Since most published results for alpha-Klotho have normal ranges of 0.8 - 2.0 ng/ml, I emailed Jinfiniti to find out why their normal ranges are so much higher : The founder She responded with:
" We use a very different assay and reference ranges are established by our own studies that have more subjects and with a variety of health conditions. Your Klotho is very low relative to what we see in most individuals. Low Klotho levels are usually associated with some health conditions in most individuals with very low Klotho. In your case, it may be related to diabetes, hypertension and related kidney suboptimal function.".
This confirms what AlexKChen quoted about antibody (ELISA) based Klotho tests not being very repeatable, so each ELISA panel may produce results that need to be scaled differently to estimate absolute ng/mL values, and those scaling factors are often wrong. So each test labs needs to generate their own reference ranges to interpret the Klotho test result.
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Have you received the longevitylabsolutions results yet? Iād rather not spend over $1,000 on the Jinfiniti for just this metric!
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Thereās a new longevity company for klotho gene therapy (Jim OāNeill is on it), idk delivery method or further details.
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I will be doing mailing in my Longevitylabsolutions sample 8/26/24.
Longevity Labs took almost 4 weeks to process the klotho test (I paid $199, but the current price is $229) and my result was 1156 pg/mL, which is at the upper end of the reference range (750-1250) for the 19-39 age group. This is exactly the opposite of the results I got from Jinfiniti on Jun-17-2024, which had me well below their reference range for youthful Klotho levels.
Here are the full reference ranges along with my test result from Longevity Labs:
SOLUBLE KLOTHO 1156 pg/mL
<548.8 is associated with 52% increased risk for all-cause mortality
<660.9 is associated with 58% increased risk for cardiovascular mortality
18-39 years: 740 ā 1250
40-59 years: 550 ā 1000
60-79 years: 350 ā 750
over 79 years: 200 - 600
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Longevity Labs disappeared, what happened?
I cannot access longevitylabsolutions.com either, but the usual website down detectors claim the website is up and so the problem is likely some blacklisting by anti-virus software or a temporary issue over the busy Black Friday weekend.
Iāve talked with Jinifinity and they will do a stand alone Alpha Klotho level and will soon be offering more range of panels. I can order it as of today including all processing/shipping ~$300. Their panel that currently has this is $1200, but has a lot of other things on it.
Longevitylabs has two klotho blood tests - one is way more expensive than the other. Is there any real difference in usefulness between the two?
sent my sample in todayā¦ now how do i get injectable klotho?
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Short answer
Longevity NutriCareās āSoluble Klotho Blood Testā is run in a CLIA-accredited laboratory (CLIA # 45D2297805, Longevity Lab Solutions, Spring TX) (www.hipaaspace.com), so the lab is legally permitted to report human diagnostic results in the United States.
That is a necessary baseline for reliabilityābut it is not sufficient. The real-world accuracy of any Ī±-klotho measurement still depends on three things you should verify before you spend $235:
| What to ask for | Why it matters | Typical red flags |
|---|---|---|
|
Assay platform & kit. ā¢ Which manufacturer (e.g., IBL-America ELISA, Finostics TRF-immunoassay, MSD electrochemiluminescence)? ā¢ Is it FDA-cleared, RUO, or an in-house LDT? |
Commercial ELISAs for klotho differ by up to an order-of-magnitude, and some show poor correlation with eGFR or each other (PubMed, PMC) | āResearch-use-onlyā kit with no validation summary; no mention of duplicate wells or calibrators. |
| Validation data. Ask for the labās summary of limit of detection, linearity, recovery, and intra-/inter-assay coefficient of variation (CV). | Good clinical labs publish or furnish these numbers (CV ā¤ 8 % intra-run; ā¤ 10 % inter-run is typical). | āProprietaryā or unavailable performance metrics. |
| Sample-handling protocol. ā¢ Venous serum vs. finger-prick-dried blood spots. ā¢ Use of EDTA tubes + protease inhibitors. ā¢ Max shipping time and temperature. | Klotho is a 130 kDa protein that degrades with freezeāthaw and proteolysis; DBS has not been formally validated for this marker. | Home finger-stick kit with no cold-chain requirement or stabilizer. |
If Longevity NutriCare cannot document those points, your result may have more analytical noise than biological signal.
How does it stack up against other options?
| Option | Regulatory status | What you get | Practical notes |
|---|---|---|---|
| Longevity NutriCare | CLIA-accredited independent lab (www.hipaaspace.com) | Home collection kit ā soluble Ī±-klotho report | Small lab (ā10 employees) (LinkedIn); kit details and assay manufacturer not disclosed on the website (longevitynutricare.com) |
| Academic reference labs (eg, ARUP, Mayo) | CLIA + CAP; large QA teams | Most do not yet list clinical Ī±-klotho; tests are offered only under IRB protocols or as research assays | |
| Research kits you run yourself | RUO only | ELISA plate, standards | You still need duplicate wells, calibrators, and a plate readerāand results remain non-diagnostic |
Bottom line
Longevity NutriCare looks legitimate from a regulatory standpoint, but the Ī±-klotho assay itself is still an emerging, high-variability biomarker.
Before ordering, insist on seeing the assay validation summary and make sure the sample-handling protocol has been stress-tested. If the lab can provide robust CVs, established reference ranges, and documentation that the home-collection method preserves klotho integrity, the test can be a useful data point. Otherwise, you may be paying for a number that is difficult to interpret or reproduce.
(If you need help formulating specific questions to send the labāor want alternative ways to monitor renal-klotho biology such as FGF-23, phosphate, and eGFR panelsājust let me know.)
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