Genistein extends median, mean, and maximum lifespan in C57BL/6J mice when started at 16 months of age.
9.3% increase in median lifespan
9.2% increase in maximum lifespan
10.0% in mean lifespan
Healthspan:
“Genistein treatment decreased the severity of multiple age-dependent phenotypes, including poor coat condition, fur color loss, and tail stiffening.”
“Genistein-fed mice manifested delayed loss of body weight and visible signs of aging.”
“Genistein treatment for 8 months reduced the levels of a large proportion of pro-inflammatory cytokines (IL-1α, IL-2, IL-6, TNF-α, CCL2, CCL5, CXCL1, and CXCL5) in aged animals.”
2.7.2. Kinetic Study of the Inhibitory Effects on the Formation of AGEs by Genistein
Since genistein was determined as the most effective natto isoflavone in trapping MGO, we further selected genistein to evaluate its kinetics of AGEs inhibition. BSA (30 mg/mL) was incubated with MGO (60 mM) in the presence or absence of genistein (200 μg/mL) in a phosphate buffer solution (0.05 mol/L PH 7.4). The reaction mixture was collected at different time points (240, 480, 720, 960, 1200, and 1440 min). Fluorescence at an excitation/emission wavelength of 370/450 nm, which is characteristic of AGEs, was used to measure AGEs levels.