2.7.2. Kinetic Study of the Inhibitory Effects on the Formation of AGEs by Genistein
Since genistein was determined as the most effective natto isoflavone in trapping MGO, we further selected genistein to evaluate its kinetics of AGEs inhibition. BSA (30 mg/mL) was incubated with MGO (60 mM) in the presence or absence of genistein (200 μg/mL) in a phosphate buffer solution (0.05 mol/L PH 7.4). The reaction mixture was collected at different time points (240, 480, 720, 960, 1200, and 1440 min). Fluorescence at an excitation/emission wavelength of 370/450 nm, which is characteristic of AGEs, was used to measure AGEs levels.