In my view another Dr Sandra Kaufmann video worth watching.
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It’s an excellent product, but a pretty bad video and injection technique. He injects too deep, that’s why he’s bleeding. Why the needle is so long? It’s a kind of dangerous and he could easily hit a nerve.
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Anyone know of any reliable sources to purchase exosomes? I’d love to experiment with topical application following microneedling both my face and scalp.
Several South Korean sites sell them. I’m not sure that they are ready for prime time yet. Some people get bad reactions from topical use.
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“The caveat to it is you’ll only have good regeneration if you’re healthy, young, and have strong regenerative signals.”
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FWIW
If you contact Sandra Kaufmann MD, she answers her emails herself. She would tell you which manufacturer/ brand she uses personally.
NOT that she will write a prescription.
She may know who?
KAUFMANNAAI@GMAIL.COM
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Has anyone done an in-depth look at the research behind the purported benefits of exosomes, and how solid the science is? Best papers on this topic?
From the Gerontological Research Group mailing list:
Stephen McCain has teamed up with supplement super-guru Sandra Kaufmann, MD to offer ultra-cheap exosome injections in Vegas on December 15 (either at-cost or very close to it). I believe the cost is $900 for 12.5 billion exosomes, $1500 for 25b, $2800 for 50b, $4000 for 75b, and $5000 for 100b.
But schedule a call with Stephen using that website and he can fill you in.
I have researched them a lot in the past. Their benefits are simlar to those of stem cells, which means they have limited benefits except when it comes to healing injuries. One main difference between exosome and stem cell treatments is that exosomes are more bioavailable than stem cells. Exosomes are highly complicated in that they basically carry a complex mix of cargo from the cells from which they were derived. So e.g. mesenchymal stem cell exosomes will have lots of cargo usually found and produced by mesenchymal stem cells. This will include growth factors, cytokines and miRNAs.
As for their benefits, I don’t think they have much potential for healthy people. Their main usefulness is similar to that of stem cells, that is to fix injuries and damaged tissues. If you don’t have anything particular like that to heal then I doubt it will have much benefit at all.
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As for their benefits, I don’t think they have much potential for healthy people. Their main usefulness is similar to that of stem cells, that is to fix injuries and damaged tissues
Uh, can’t stem cells transplant themselves into the niche?
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People say “increased energy”. One said “increased sex drive” (or things associated with growth hormone) [this is not good]. Some people say improved quality of skin/less wrinkling/increased hair growth [this probably correlates with injury healing].
I decided “fuck it” and I’ll go for one set of infusions… But most of my issues are brain related rather than “rest of body” related.
Also one person reported a drastic reduction in DNAm age by ~20 years (though that was temporary)
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Yes they can transplant themselves into the niche, but they don’t usually do that to a significant extent except in tissues that are constantly being turned over or perhaps in cases when there are some cells that are damaged and dead and left space for new ones to replace them.
If we’re talking about some post-mitotic cells in a tissue the niche will already be full of cells and stem cells that arrive there won’t create new tissue in the niche because the niche is already full. Only if some of the cells die will there be space for the stem cells to replace them.
A lot of times when injected stem cells have benefits the benefits are derived not from the cells settling in the tissue but from the exosomes the stem cells secrete. The exosomes can influence the tissue and help it repair itself. However I’m not aware of this being of benefit for cells that are already healthy.
As for the reports of benefits from exosome treatments. That’s very intereseting. I’m not sure what would explain these reports you mention. My buest guess for anecdotes like “increased eneregy” would be that the exosomes reduced inflammation in the body and brain, which in turn made the person feel they have more energy. Exosomes have been found to have strong anti-inflammatory effects in some cases.
I tried 3 days ago already (smallest evovex exosomes), I don’t feel too much different yet…
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The quantity matters.
this video below is 374b exosomes (but also from young adults, who aren’t exactly the healthiest people)
Commented on THIS Therapy Slows Down The Aging Clock By 31% - Successful Clinical Cases
10B is way way too small to feel effects. Damnit.
there are 10 trillion cells in the body
10 billion exosomes is nothing
https://isevjournals.onlinelibrary.wiley.com/doi/full/10.1002/jex2.46
if it’s 200 billion exosomes, that’s still 0.02 exosomes per cell
for analogy,
" After ingesting 50mg of melatonin, there would be approximately 3,484,625 melatonin molecules per cell in the human body, assuming an even distribution across all cells"
" After ingesting 20mg of Adderall, there would be approximately 2,394,523 Adderall molecules per cell in the human body, assuming an even distribution across all cells"
" After ingesting 100 micrograms of LSD, there would be approximately 5,005 LSD molecules per cell in the human body, assuming an even distribution across all cells"
Anyone read this book yet?
Lots of videos and presentations from Dr. Park on his YouTube channel: (though I’m skeptical of telomerase as being a significant contributor to lifespan improvement). I wonder if this guy is just jumping from one buzzword / hot topic in aging, to the next…
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Not detailed enough on the biochemistry details even on simple metrics like exosome count and composition, too wordy
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Human Exosomes Harvested from Stem Cells in Urine Produce Rejuvenation in Mice
Exosomes are a class of extracellular vesicle, membrane-wrapped packages of molecules that carry a sizable fraction of the chemical communications that takes place between cells. The various types of extracellular vesicle are presently ordered in a taxonomy by their size rather than any more subtle combination of features. Those subtle features definitely exist; exosomes from different cell types and different environmental circumstances are quite different from one another in any number of ways. The present taxonomy of extracellular vesicles is indicative of a lack of detailed understanding regarding (a) the mechanisms determining formation of extracellular vesicles, as well as (b) the factors determining the contents of extracellular vesicles.
The stem cell therapies that have long been available via medical tourism, and were later approved by regulators, are slowly morphing into exosome therapies. Extracellular vesicles are more easily harvested, stored, and delivered than is the case for cells. Since transplanted cells die quickly, the benefits of first generation stem cell therapies, such as months-long reductions in chronic inflammation, are mediated by cell signaling, such as the release and uptake of extracellular vesicles. Exosome therapies are now broadly available in overseas clinics, and are working their way into the more heavily regulated medical system. It is even possible to purchase amniotic fluid derived exosomes from providers in the US, provided one has a physician who agrees to accept delivery and make use of them.
The approach to exosome therapy noted in today’s open access paper is an interesting one. The source of cells is those that are shed into urine. When humans are the donors, this is a good way to obtain enough material for a mouse study, but a scaling process would have to be put in place for use in human patients. That means either a great deal of harvesting from many donors, or the more challenging approach of developing a well-managed cell line that can produce exosomes in bulk.
Extracellular vesicles from human urine-derived stem cells delay aging through the transfer of PLAU and TIMP1
Transplantation of young and healthy stem cells has been shown to increase health and lifespan in aged mice. A study has reported that the intraperitoneal injection of muscle stem/progenitor cells from young mice can extend healthspan and lifespan in progeroid mice. Interestingly, the transplanted cells are not detected in many rejuvenated tissues, suggesting that their anti-aging effects are mainly mediated by activating endogenous cells in the host through paracrine factors.
Secretion of extracellular vesicles (EVs) is a part of normal physiology in both prokaryotes and eukaryotes. EVs are selectively enriched with various molecules such as proteins and nucleic acids from their parent cells and serve as a key mediator of cell paracrine action by transferring these molecules to their recipient cells. Stem cells-derived EVs have become an attractive option for therapeutic uses because these nanoparticles have fewer safety concerns and are easy to store, transport, and use compared with stem cells themselves. Recent studies have reported that EVs from embryonic stem cells, induced pluripotent stem cells, adipose stem cells, hypothalamic stem/progenitor cells, and umbilical cord- or umbilical cord blood-derived mesenchymal stem cells (MSCs) can alleviate aging-related phenotypes in aged mice, indicating the promising potential of EVs as an anti-aging agent. Nevertheless, the use of these stem cells as the “factory” to harvest EV are limited by many problems, such as the ethical issue for cell use, the lack of enough source to obtain cells, or/and the requirement of fast, convenient, and invasive procedures for cell isolation.
As compared with stem cells from other sources, urine-derived stem cells (USCs) can be collected by a low-cost, simple, and safe method without ethical concerns. We have previously demonstrated that the local injection of USC-derived EVs (USC-EVs) can accelerate wound repair in diabetic mice by enhancing angiogenesis. We have also found that the intravenous injection of USC-EVs can reduce bone loss and enhance bone strength in osteoporotic mice. Moreover, these nanovesicles can exert protective effects against glucocorticoid-induced osteonecrosis by promoting angiogenesis, and suppress cell apoptosis after systemic administration. In our previous study, we obtained proteomic data regarding the differentially expressed proteins between USC-EVs and USCs. In this study, we further analyzed these data and found that a class of USC-EVs-enriched proteins have been previously shown to possess anti-aging function, such as tissue inhibitor of metalloproteinases 1 (TIMP1), plasminogen activator urokinase (PLAU), insulin-like growth factor binding protein 5, senescence marker protein-30, and connective tissue growth factor. Thus, we hypothesized that USC-EVs might be capable of rejuvenating old organs from aging via transferring of anti-aging proteins.
Here, we tested the effects of USC-EVs on cellular senescence in vitro and on the aging-related phenotypes in different organs of both senescence-accelerated mice and natural aging mice. The intravenous injection of USC-EVs improves cognitive function, increases physical fitness and bone quality, and alleviates aging-related structural changes in different organs of senescence-accelerated mice and natural aging mice. The anti-aging effects of USC-EVs are not obviously affected by the USC donors’ ages, genders, or health status. Proteomic analysis reveals that USC-EVs are enriched with PLAU and TIMP1. These two proteins contribute importantly to the anti-senescent effects of USC-EVs associated with the inhibition of matrix metalloproteinases, cyclin-dependent kinase inhibitor 2A (P16INK4a), and cyclin-dependent kinase inhibitor 1A (P21cip1). These findings suggest a great potential of autologous USC-EVs as a promising anti-aging agent by transferring PLAU and TIMP1 proteins.
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This is effectively what I had in my knee. I think this kind of treatment is going to become a lot more popular and accessible. I believe Missouri may have legalized the use of it, although I may have my facts wrong.
FWIW
My view
If you would like to get a basics understanding on the mechanism of action of exosomes The book is worth listening {I have the audiobook version]
Most people have no understanding how exosomes work.
I thought the latest trend was actually to move to lab made exosomes. A friend of mine used exosomes from Kimera Labs by a doctor in Thailand to activate her natural fibroblasts to improve collagen. Results are really impressive with a more radiant skin with less wrinkles. Different exosomes can be used for non-skin related improvements of effects of aging.
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