D-allulose was found to be more reactive than fructose and glucose in glycation reactions.[15]
Rare sugars are defined as monosaccharides and their derivatives that are rarely observed in nature. Most popular rare sugars are D-allulose, D-tagatose, D-allose, and D-sorbose. D-Allulose (also known as D-psicose) is the C-3 epimer of fructose. It is not commonly found in nature and exists in small amounts in the leaves of Itea plant (Zuina), wheat, and some bacteria (Hossain, Yamaguchi, Matsuo, Tsukamoto, & Toyoda, 2015). Since D-allulose is also a reducing sugar, it undergoes Maillard reaction when heated with proteins and produces enhanced functional properties (Hossain et al., 2015). Glycation of α-lactalbumin with D-allulose, fructose, and glucose was investigated and it was found that Maillard reaction rate, antioxidant activity, and the browning degree was the highest at D-allulose modified proteins (Sun et al., 2006; Sun, Hayakawa, Ogawa, Fukada, & Izumori, 2008).
Microwave heating is an alternative to the conventional glycation process since it provides higher heating rates with shorter times. There are few studies about microwave glycation of proteins in the literature and most of the studies concluded that microwave glycation was an effective way for protein modification (Bi et al., 2015; Guan et al., 2011; Nasrollahzadeh, Varidi, Koocheki, & Hadizadeh, 2017). Soy protein isolate (SPI) glycation with microwave heating by using lactose, maltose, dextran and soluble starch as sugar sources revealed that use of microwaves enhanced the mobility of sugar molecules, therefore, increased the reaction rate (Guan, Qiu, Liu, Hua, & Ma, 2006). Moreover, another study reported that the microwave irradiation could be the reason for the increase of water exposure to hydrophobic core residues of protein and breaking of the disulfide bonds, thus increasing the reaction rate (Guan et al., 2011).
======
The addition of d-allulose can make products produce a stronger water holding capacity in foods compared with that of sucrose. Soy gels with added d-allulose have a remarkable impact on digestive behavior, a property that can be used to design low-calorie, satiety enhancing confectionery products [62,63]. Unlike sucrose and sorbitol, heating of myofibrillar protein with d-allulose facilitates the formation of both disulfide and non-disulfide crosslinks, which may be related to the mechanical properties and water holding capacity of d-allulose gels [64]. Functional foods and formulas for special medical purposes that utilize d-allulose in the Maillard reaction with proteins are effective in the prevention of dental caries and related diseases caused by oxidative stress. A recent longitudinal study on d-allulose, in which the lifespan of Caenorhabditis elegans was increased under both monogenic and axenic culture conditions, found increased resistance to oxidative stress by d-allulose through a dietary restriction mechanism [65]. These results suggest that d-allulose is an excellent candidate for dietary restriction mimetics.