I have old skin and it has seen a lot of sun. I watch this topic with interest and have tried oneskin and a few others just to see what they do. Not that I care very much, but I like to try things.
I recently bought a bottle of Tremella mushroom capsules. Tremella was of interest when I was starting to grow mushrooms, but it’s a little harder than most because it’s a fungus that grows on another fungus and I’m not that good at just one fungus so never gave it a try. It has been used for thousands of years as a skin moisturizer and is supposed to be able to hold 500 times it weight in water.
I think the capsules work better than anything I’ve tried. My skin is very smooth. I bought this from @Joseph_Lavelle and forgot the web site but it’s not that expensive.
I’m very limited in the testing I can get done here for a reasonable price. So I’m more interested in the visible/physical effects and some basic measurements I can do at home.
For osteo, clearing out senescent cells appears to be one way to reduce inflammation that may be closely tied to that condition.
What is interesting about senescent cells is how many conditions they play a part in. I tell people to pick their favorite disease/condition and do a google search like this > heart disease senescent cells <> osteoarthritis senescent cells < just tack on > senescent cells < to any condition and you may be surprised.
I’ve been “killing” my senescent cells for about 4 years but the method I’ve been using is tissue specific and does not appear to fully address the tissues involved in OA. One of the reasons I’m about to try an interesting peptide called FOXO4-DRI in January. I have arthritis in my left hand, and my little finger is where it’s most prevalent, so I’m interested to see if FOXO4-DRI helps.
Asking Google AI > osteoarthritis senescent cells < I get this answer.
" AI Overview
Yes, senescent cells are a key factor in the development of osteoarthritis (OA). Senescent cells are cells that have undergone a process of cellular senescence, which can occur in many tissues, including cartilage, synovium, and subchondral bone. In OA, senescent cells contribute to the disease in a number of ways, including:
Creating an inflammatory environment: Senescent cells secrete cytokines that impact the immune system and can contribute to inflammation.
Altering the synovial microenvironment: Senescent cells can alter the synovial microenvironment, which can lead to OA-like arthropathy.
Degrading cartilage: Senescent cells can contribute to cartilage degradation.
Some markers of cellular senescence include: Beta-galactosidase expression, Telomere length, Mitotic activity, and Senescence-associated secretory phenotype (SASP).
This is interesting! My skin is as dry as the Sahara. For the first time, I just ordered hyaluronic acid in pill form to see if I could moisturize from the inside out.
Is that the effect it had on your skin? Or did you mean something else when you said it helped smooth your skin? (Aside from dehydration, my skin is pretty good).
Fullscript is correct. I am a little busy and just didn’t have time to look it up.
All the rough areas become smooth. It’s crazy how fast it works. I’m interested in finding out how it works on other people. Most of my problems seem to come from my skin being so thin and that seems to cause wrinkles too. There’s no plump. This seems to help noticeably.
Thanks, I thought I’d been cleaning up senescent cells with Rapamycin. I’ve been taking it for over 2 years and generally feel fantastic, except for my worsening hands. Will you do this FOXO experiment with or without Rapa?
Re tests, yes I’m Canadian too but planning to get some tests, including Rapamycin levels, done in San Diego when I visit there soon. If anyone has a lab to recommend, let me know. Labcorp had a heckuva time dealing with my lack of US address even though I used my son’s.
While Rap does have senolytic properties and inhibits SASP, I do not think it is the best nor the most effective senolytic.
I will continue to do our Rapa cycle as we introduce FOXO4-DRI. My approach is to maintain my baseline. Our baseline currently includes a lot of things so I like to leave that “as is” when I add or try something new unless there is a conflicting issue.
I have not read enough to fully understand how effective Rap is nor what the effective dose is for it to act effectively on senescent cells. When it comes to senolytics, dose often critical. For example Quercetin is not a senolytic at low does. What is a low dose of Q? under 2000 to 3000mg it’s not very good at all forthat purpose but it’s beneficial at low 500mg doses for other things.
Rapa is more of an inhibitor of senescence. But again, at what dose? and in humans or mice?
AI Overview
Yes, rapamycin has senolytic-like properties:
Senescence suppression
Rapamycin can suppress senescence and the senescence-associated secretory phenotype (SASP).
Cellular senescence inhibition
Rapamycin can inhibit cellular senescence in multiple cell types, including fibroblasts.
Senescence delay
Rapamycin can delay entry into senescence in cells treated with chemical stress, ionizing radiation, and other factors.
Senescent cell reduction
Rapamycin can reduce senescent cell subpopulations.
Here is an article by Mikhail V Blagosklonny whom most of us taking Rap are aware of.
Aging is characterized by a gradual decline in function, partly due to accumulated molecular damage. Human skin undergoes both chronological aging and environmental degradation, particularly UV-induced photoaging. Detrimental structural and physiological changes caused by aging include epidermal thinning due to stem cell depletion and dermal atrophy associated with decreased collagen production. Here, we present a comprehensive single-cell atlas of skin aging, analyzing samples from young, middle-aged, and elderly individuals, including both sun-exposed and sun-protected areas. This atlas reveals age-related cellular composition and function changes across various skin cell types, including epidermal stem cells, fibroblasts, hair follicles, and endothelial cells. Using our atlas, we have identified basal stem cells as a highly variable population across aging, more so than other skin cell populations such as fibroblasts. In basal stem cells, we identified ATF3 as a novel regulator of skin aging. ATF3 is a transcriptional factor for genes involved in the aging process, with its expression reduced by 20% during aging. Based on this discovery, we have developed an innovative mRNA-based treatment to mitigate the effects of skin aging. Cell senescence decreased 25% in skin cells treated with ATF3 mRNA, and we observed an over 20% increase in proliferation in treated basal stem cells. Importantly, we also found crosstalk between keratinocytes and fibroblasts as a critical component of therapeutic interventions, with ATF3 rescue of basal cells significantly enhancing fibroblast collagen production by approximately 200%. We conclude that ATF3-targeted mRNA treatment effectively reverses the effects of skin aging by modulating specific cellular mechanisms, offering a novel, targeted approach to human skin rejuvenation.
ATF3 protein levels are also increased (in liver and fibroblasts, at least) in a number of long-lived mice (rapa, acarb, CR, and methionine restricted).
I have seen versions of this system for sale in a few places, many much cheaper. It does look promising. I might try this. But one thing, how do you make sure the serum is really going out if the bottle is pointing up or horizontal? Lools loke you would need to do this with ones head highly elevated or lying down.
I a micro needling once a month. Awhile ago i dismissed derma rollers because of the mechanical problem that they tear as much as pierce when the roll. But also I was worried about keeping them clean. I dump my needle cartridge after every session.
You give the old mercury thermometer reset shake, that forces the serum out of the bottle and on to the needles. I do that before each row.
I started using the system once a week. I have no issues with any recovery next day, I think my skin maybe a bit thicker that needle size is design for.
They recently added Copper Peptide GHK-Cu to their serum selection, there is a bunch left over in a bottle after you are done so I will use it on top of my head - it supposed to help with hair growth as well.
BTW, we always talk about treating the face directly but SCALP thinning is a big part of sagging face. There are just not enough products for scalp skin treatment. Treating scalp is hard because of the hair as well.
How to nurture your microbiome to look after your skin
Our skin is host to a thriving community of bacteria, some of which help to restore and protect our epidermis. The hunt is now on for treatments that make the most of these allies
Beneficial skin bacteria could also be the key to maintaining a smooth, wrinkle-free appearance. Our skin is like a fortress constructed from layers of skin cells packed together. In between the cells are lipids that keep the skin supple and plump, and certain bacterial species help to replenish those stores.
“Cutibacterium stimulates the skin to produce sebum, which protects the skin, reduces water loss and increases hydration,” says Holly Wilkinson, who studies wound healing at the University of Hull, UK. Staphylococcus epidermidis and Streptococcus thermophilus can produce ceramides, meanwhile, which can also reduce water loss and protect the skin barrier.
