I guess that if we get effects from lower doses, then the translation to mammals and primates might be easier achieved. Due to reduced risk for severe sideeffects.
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Yes, true, thatās a important part also but I would like to have that as a good next step. Currently Iām very curious to see if we can push the lifespan needle a bit more. If we succeed in motr than doubling the lifespan of a worm that would be a really great first step which I think can increase the interest in the field.
So I have change the dosing now to:
Doxycycline Hcl (15uM)
Doxycycline hyclate (15uM)
GSK2126458 (10uM) + Doxycycline Hcl (15uM)
GSK2126458 (10uM) + Doxycycline hyclate (15uM)
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For anyone else wondering about why their results donāt show any lifespan extension due to rapamycin:
For example, rapamycin, a popular lifespan-extending drug candidate, has been shown to extend lifespan across different species including C. elegans[18,34]. However, other groups, including the CITP, which specifically tests compounds in C. elegans in three different sites to thoroughly interrogate a candidate, have similarly not reproduced lifespan extension by rapamycin in C. elegans[5]. Other groups have reported recently that the reason may be that rapamycin precipitates out of the agar, something we have also observed, depending on concentrations.
Makes me wonder if some of the drug combination results in C. elegans have to do with solubility (one of the drugs improving solubility of rapamycin compared to rapamycin alone). Also makes me wonder about wormbot results involving rapamycin.
There was a combination with rapamycin that got tested 3 times already. In interventions browser there is a dropdown to look at them separately.
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it will be interesting to see what Doxycycline Hcl (15uM) will do. hopefully that dose will be better than the Doxycycline HCl 50ĀµM, that I sponsored.
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Ah, thanks for pointing that out! It would also be interesting to start the treatment a bit later in life to see if that improves the lifespan effect. One day I will buy a wormbot and have it in my basement and do lots of iteration quickly and for philantropic purposes 
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Killifish might be better.
Valenzano lab investigates the evolutionary and ecological causes responsible for the vast diversity of aging rates and lifespans across species in nature.
We promote around the world the access of African killifish as a laboratory model by training labs for killifish husbandry, developing and sharing husbandry protocols, sharing laboratory and wild-derived killifish strains and resources for this species.
We promote a curiosity-driven, inclusive and open lab culture, supporting a diverse and dynamic research environment. We share our code, data and results.
We strive to generate valuable scientific knowledge, tools and resources that can be accessed, replicated and used broadly by other members of the scientific community and of the society.
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How about 500 % increase? As we have seen in the ITP there are synergies between TOR pathway and insulin signalling.
āThe new research uses a double mutant in which the insulin signaling (IIS) and TOR pathways have been genetically altered. Because alteration of the IIS pathways yields a 100 percent increase in lifespan and alteration of the TOR pathway yields a 30 percent increase, the double mutant would be expected to live 130 percent longer. But instead, its lifespan was amplified by 500 percent.ā
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Alex is a member here, but he seems to have been too busy to visit the past year. Its a little more complex than just finding some rich guys to fund it. You need first to find the research lab / people to lead it. Rich Miller / ITP would be naturals for this (at some level) but the NIA isnāt funding them well and Rich Miller isnāt predisposed to taking money from just anyone (e.g. heās not interested in Saudi money). The Wormbot guys would be a natural, but Iām sure with their Matt Kaeberlein connections they have lots of access to rich guysā¦ (Mattās new company includes Ex-microsoft, and Real Estate money from the Pacific Northwest.