# Ideas on Protocols for Testing Higher Rapamycin Doses **URL:** \ **Category:** General\ **Created:** [December 17, 2021, 7:26pm UTC](https://www.rapamycin.news/t/ideas-on-protocols-for-testing-higher-rapamycin-doses/400 "2021-12-17T19:26:38Z")\ **Posts on this page:** 9\ **Page:** 4
**Author:** ![RickCohenMD](https://www.rapamycin.news/letter_avatar_proxy/v4/letter/r/48db29/32.png) [@RickCohenMD](https://www.rapamycin.news/u/RickCohenMD)\ **Post date:** [December 21, 2022, 7:12pm UTC](https://www.rapamycin.news/t/ideas-on-protocols-for-testing-higher-rapamycin-doses/400/61 "2022-12-21T19:12:18Z")
A simple method is just track a fixed power/heart rate curve. For example what is your HR at steady state 125w. Then reduce to 50W and see how long it takes for HR to reduce to 80%, ---
**Author:** ![Joseph](https://www.rapamycin.news/letter_avatar_proxy/v4/letter/j/dc4da7/32.png) [@Joseph](https://www.rapamycin.news/u/Joseph)\ **Post date:** [December 21, 2022, 8:36pm UTC](https://www.rapamycin.news/t/ideas-on-protocols-for-testing-higher-rapamycin-doses/400/62 "2022-12-21T20:36:22Z")
> [@RickCohenMD](#): > > “As a physician I have negotiated pricing for sirolimus including lab draw free at LabCorp for $49.” Could this pricing{$49.00 per draw] be available/made available to member’s/users on this forum? ---
**Author:** ![RickCohenMD](https://www.rapamycin.news/letter_avatar_proxy/v4/letter/r/48db29/32.png) [@RickCohenMD](https://www.rapamycin.news/u/RickCohenMD)\ **Post date:** [December 21, 2022, 9:16pm UTC](https://www.rapamycin.news/t/ideas-on-protocols-for-testing-higher-rapamycin-doses/400/63 "2022-12-21T21:16:11Z")
Just to confirm that is 37.6 ng/ml? Not PPM. Is so that is a very good level. Did you take GFJ prior to or with the sirolimus? What concentration? 1 or 2 mg. What formulation did you use? Pfizer Rapamune or Generic. If so know the brand? Dr. Reddys is triangular with number 53 or 54. All these details make a difference. ---
**Author:** ![RapAdmin](https://www.rapamycin.news/user_avatar/www.rapamycin.news/rapadmin/32/377_2.png) [@RapAdmin](https://www.rapamycin.news/u/RapAdmin)\ **Post date:** [December 21, 2022, 10:24pm UTC](https://www.rapamycin.news/t/ideas-on-protocols-for-testing-higher-rapamycin-doses/400/64 "2022-12-21T22:24:53Z")
> [@Joseph](#): > > Could this pricing{$49.00 per draw] be available/made available to member’s/users on this forum? One of our members found this test for $56 (a total of $64 for the total service): [Inexpensive Serum Blood Test for Rapamycin](https://www.rapamycin.news/t/inexpensive-serum-blood-test-for-rapamycin/3864) ---
**Author:** ![Bicep](https://www.rapamycin.news/letter_avatar_proxy/v4/letter/b/13edae/32.png) [@Bicep](https://www.rapamycin.news/u/Bicep)\ **Post date:** [December 22, 2022, 4:26am UTC](https://www.rapamycin.news/t/ideas-on-protocols-for-testing-higher-rapamycin-doses/400/65 "2022-12-22T04:26:07Z")
![Rapa result](https://www.rapamycin.news/uploads/default/original/2X/6/65e14e9a614626c779652a137ec93f4c4adf4957.jpeg) I did drink GFJ, probably 24 oz. Otherwise I think I was fasting that day and it was first thing in the morning. It was for sure Generic and almost certainly Biocon. Rapacon. Just a line across the top of the tablet. 1mg. I had some other generics at the time from Wal Mart, and it’s been awhile, but I’m pretty sure. I think I wrote about it on here and probably said. I don’t think there is any difference in these tablets and I’ve used several kinds. They all give me clues that tell me I’m getting the real thing. I just tested because I like numbers. ---
**Author:** ![RickCohenMD](https://www.rapamycin.news/letter_avatar_proxy/v4/letter/r/48db29/32.png) [@RickCohenMD](https://www.rapamycin.news/u/RickCohenMD)\ **Post date:** [December 22, 2022, 4:30pm UTC](https://www.rapamycin.news/t/ideas-on-protocols-for-testing-higher-rapamycin-doses/400/68 "2022-12-22T16:30:31Z")
Saliva won’t work for sirolimus. But I would be happy with an at-home microtube draw and stoked for bloodspot. These are doable but need more people checking levels so we can go to a lab and offer at least a few hundred or more samples a month to make it worth their while to update systems. ---
**Author:** ![RickCohenMD](https://www.rapamycin.news/letter_avatar_proxy/v4/letter/r/48db29/32.png) [@RickCohenMD](https://www.rapamycin.news/u/RickCohenMD)\ **Post date:** [December 22, 2022, 4:38pm UTC](https://www.rapamycin.news/t/ideas-on-protocols-for-testing-higher-rapamycin-doses/400/69 "2022-12-22T16:38:23Z")
Yes both purity and efficacy need to be confirmed. I am working with a vetted supplier of nanoparticle sirolimus in Asia that has 3rd party testing and we will be sending the final ingredients to a lab in Israel to do pharmacokinetics. Price should be less than $2 mg. This would be available without an RX. ---
**Author:** ![RickCohenMD](https://www.rapamycin.news/letter_avatar_proxy/v4/letter/r/48db29/32.png) [@RickCohenMD](https://www.rapamycin.news/u/RickCohenMD)\ **Post date:** [December 22, 2022, 6:44pm UTC](https://www.rapamycin.news/t/ideas-on-protocols-for-testing-higher-rapamycin-doses/400/71 "2022-12-22T18:44:56Z")
For labs or the meds? We want to run 4 more people comparing levels to generic and then send the samples to Israel for full analysis. It will be a few months. I’ll let people know. ---
**Author:** ![Daph](https://www.rapamycin.news/letter_avatar_proxy/v4/letter/d/ac91a4/32.png) [@Daph](https://www.rapamycin.news/u/Daph)\ **Post date:** [April 6, 2024, 10:53pm UTC](https://www.rapamycin.news/t/ideas-on-protocols-for-testing-higher-rapamycin-doses/400/72 "2024-04-06T22:53:05Z")
> [@RapAdmin](#): > > > Any sign of a drop in lymphocytes or an increase in CRP or other inflammatory markers is a big red flag that dose is too high. > > > > Doses for aging should not be immunosuppressive, and should not be high enough to be disrupting the balance of cytokines (so-called “sirolimus-induced inflammatory syndrome”). > > > > It has been documented in certain cases that rapamycin can induce a significant inflammatory attack on the body. The term for it is “Sirolimus-Induced Inflammatory Syndrome”. [This paper](https://www.ncbi.nlm.nih.gov/labs/pmc/articles/PMC3538748/?fbclid=IwAR0olH8zmCUDvd3d_3C_dVbxvUAl9OOPogR2bUhMpNthIiahoOFEjIbcBBU) explores why it happens and the mechanisms involved. Low doses of rapamycin in the range of 4-6 mg/week, or 1 mg every other day, can also induce an inflammatory syndrome. Unfortunately it did with me, starting with transient pruritis and joint pains, and ending with the exacerbation of an immune-related myocarditis condition that I was taking it to prevent. I had new and more frequent heart pain - once after a weekly dose of 4 mg and once after a 6 mg dose. Then after switching to 1 mg every other day, I had new mild, but continual heart discomfort with new left shoulder pain on and off for 2.5 weeks. Toward the end of that episode, I ran some blood tests and found by CRP had shot up from 0.8 to 25.4. I also had low hemoglobin and hematocrit, and a high neutrophils/Lymphocytes ratio of 3.1. So that’s the end of that experiment, for now. It’s possible that this happened because I have an autoimmune condition and/or my immune system is dysfunctional in some other way. It could be that that I need to take it more slowly, that the rapamycin can still have a beneficial effect on my immune system and help in the long run, for example, by increasing Tregs per this study involving people with rheumatoid arthritis: [Low-Dose Sirolimus Immunoregulation Therapy in Patients with Active Rheumatoid Arthritis: A 24-Week Follow-Up of the Randomized, Open-Label, Parallel-Controlled Trial - PMC](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6874993/). Then again, I found this study in mice showing that low dose, but not high dose, rapamycin can exacerbate and prolong autoimmune disease: [pubmed.ncbi.nlm.nih.gov](https://pubmed.ncbi.nlm.nih.gov/22574188/) Then there is the paper you cited about sirolimus-induced inflammatory syndrome. I am not ready to give up so will take a break for a couple of months and may try again, possibly with lower dosing and longer intervals, while monitoring my inflammatory markers more frequently. [Previous page](https://www.rapamycin.news/t/ideas-on-protocols-for-testing-higher-rapamycin-doses/400.md?page=3)