# Antibiotics that target mitochondria extend lifespan in C. elegans **URL:** \ **Category:** General\ **Created:** [April 20, 2024, 11:39pm UTC](https://www.rapamycin.news/t/antibiotics-that-target-mitochondria-extend-lifespan-in-c-elegans/13463 "2024-04-20T23:39:16Z")\ **Posts on this page:** 12\ **Page:** 1
**Author:** ![Joseph](https://www.rapamycin.news/letter_avatar_proxy/v4/letter/j/dc4da7/32.png) [@Joseph](https://www.rapamycin.news/u/Joseph)\ **Post date:** [April 20, 2024, 11:39pm UTC](https://www.rapamycin.news/t/antibiotics-that-target-mitochondria-extend-lifespan-in-c-elegans/13463/1 "2024-04-20T23:39:16Z")
> **[Antibiotics that target mitochondria extend lifespan in C. elegans - PubMed](https://pubmed.ncbi.nlm.nih.gov/37950722/)** > > Aging is a continuous degenerative process caused by a progressive decline of cell and tissue functions in an organism. It is induced by the accumulation of damage that affects normal cellular processes, ultimately leading to cell death. It has been... PDF copy of the paper; [aging-v15i21-205229.pdf](https://www.rapamycin.news/uploads/short-url/peqbr8TY0GX6PN1kwMu1GdUFwaV.pdf) (1.4 MB) ---
**Author:** ![Goran](https://www.rapamycin.news/letter_avatar_proxy/v4/letter/g/a6a055/32.png) [@Goran](https://www.rapamycin.news/u/Goran)\ **Post date:** [April 21, 2024, 3:08am UTC](https://www.rapamycin.news/t/antibiotics-that-target-mitochondria-extend-lifespan-in-c-elegans/13463/2 "2024-04-21T03:08:19Z")
So this is basically the DAV protocol, some of us on this board has gone through. ---
**Author:** ![Joseph](https://www.rapamycin.news/letter_avatar_proxy/v4/letter/j/dc4da7/32.png) [@Joseph](https://www.rapamycin.news/u/Joseph)\ **Post date:** [April 21, 2024, 3:16am UTC](https://www.rapamycin.news/t/antibiotics-that-target-mitochondria-extend-lifespan-in-c-elegans/13463/3 "2024-04-21T03:16:51Z")
Yes, it is. Michael P Lisanti is one of the authors of the paper, he is the Chair of; Translation Medicine, School of Science, Engineering and Environment (SEE) University of Salford, Greater Manchester M5 4BR, UK. ---
**Author:** ![adssx](https://www.rapamycin.news/user_avatar/www.rapamycin.news/adssx/32/14464_2.png) [@adssx](https://www.rapamycin.news/u/adssx)\ **Post date:** [April 21, 2024, 6:20am UTC](https://www.rapamycin.news/t/antibiotics-that-target-mitochondria-extend-lifespan-in-c-elegans/13463/4 "2024-04-21T06:20:40Z")
Worth doing in the Ora Biomedical Challenge to see if we can reproduce the findings. ---
**Author:** ![A\_User](https://www.rapamycin.news/user_avatar/www.rapamycin.news/a_user/32/40991_2.png) [@A\_User](https://www.rapamycin.news/u/A_User)\ **Post date:** [April 21, 2024, 6:59am UTC](https://www.rapamycin.news/t/antibiotics-that-target-mitochondria-extend-lifespan-in-c-elegans/13463/5 "2024-04-21T06:59:12Z")
> [@Goran](#): > > So this is basically the DAV protocol If you read the paper you will see that the ‘protocol’ decreases median lifespan in that study. This has been discussed here as well: > [@My DAV\* Therapy Experiment begins! \*Doxycycline, Azithromycin and Vitamin C](https://www.rapamycin.news/t/my-dav-therapy-begins-doxycycline-azithromycin-and-vitamin-c/9619/424): > > I commend their effort and hope they continue to research this, I just hate “movements” that do not allow people to publish possible negatives of any therapy, it so unpersuasive. However, DAV decreased median lifespan, and in isolation doxy was much more effective. [image] [image] One component of this, Azithromycin increases death rate and cancer relapse in a randomized controlled trial that had to be stopped early (ALLOZITHRO Randomized Clinical Trial). There are those who think this can’t generalize to others, there is also an association of increase in all-cause mortality and cardiovascular death. [https://pubmed.ncbi.nlm.nih.gov/28787506/](https://pubmed.ncbi.nlm.nih.gov/28787506/) > [@My DAV\* Therapy Experiment begins! \*Doxycycline, Azithromycin and Vitamin C](https://www.rapamycin.news/t/my-dav-therapy-begins-doxycycline-azithromycin-and-vitamin-c/9619/124): > > Taking two antibiotics based on a patent and not published clinical research is not a no brainer. Any possible negative effect on the microbiome aside, azitromycin is associated with an increased risk of sudden cardiovascular death. During 5 days of therapy, patients taking azithromycin, as compared with those who took no antibiotics, had an increased risk of cardiovascular death (hazard ratio, 2.88; 95% confidence interval [CI], 1.79 to 4.63; P\<0.001) and death from any cause (hazard ratio, 1.85; 95% CI, 1.25 to 2.75; P=0.002). Patients who took amoxicillin had no increase in the risk of death during this period. Relative to amoxicillin, azithromycin was associated with an increased risk of cardiovascular death (hazard ratio, 2.49; 95% CI, 1.38 to 4.50; P=0.002) and death from any cause (hazard ratio, 2.02; 95% CI, 1.24 to 3.30; P=0.005), with an estimated 47 additional cardiovascular deaths per 1 million courses; patients in the highest decile of risk for cardiovascular disease had… ---
**Author:** ![Curious](https://www.rapamycin.news/user_avatar/www.rapamycin.news/curious/32/9492_2.png) [@Curious](https://www.rapamycin.news/u/Curious)\ **Post date:** [April 21, 2024, 7:47am UTC](https://www.rapamycin.news/t/antibiotics-that-target-mitochondria-extend-lifespan-in-c-elegans/13463/6 "2024-04-21T07:47:07Z")
I sponsor doxy in the Ora Biomedical Challenge. But the combination Doxy + Azitro did not do well. [Million Molecule Challenge Results and Leaderboard – Ora Biomedical, Inc.](https://orabiomedical.com/mmcleaderboard/) ---
**Author:** ![A\_User](https://www.rapamycin.news/user_avatar/www.rapamycin.news/a_user/32/40991_2.png) [@A\_User](https://www.rapamycin.news/u/A_User)\ **Post date:** [April 21, 2024, 7:56am UTC](https://www.rapamycin.news/t/antibiotics-that-target-mitochondria-extend-lifespan-in-c-elegans/13463/7 "2024-04-21T07:56:29Z")
What was the dose of the Doxy + Azithromycin in MMC compared to the paper? Do you get to pick the dose chosen? ---
**Author:** ![Curious](https://www.rapamycin.news/user_avatar/www.rapamycin.news/curious/32/9492_2.png) [@Curious](https://www.rapamycin.news/u/Curious)\ **Post date:** [April 21, 2024, 9:48am UTC](https://www.rapamycin.news/t/antibiotics-that-target-mitochondria-extend-lifespan-in-c-elegans/13463/8 "2024-04-21T09:48:13Z")
In the MMC the dosing was: Doxycycline HCl 50µM / Azithromycin 50µM ---
**Author:** ![jnorm](https://www.rapamycin.news/user_avatar/www.rapamycin.news/jnorm/32/35518_2.png) [@jnorm](https://www.rapamycin.news/u/jnorm)\ **Post date:** [April 21, 2024, 2:21pm UTC](https://www.rapamycin.news/t/antibiotics-that-target-mitochondria-extend-lifespan-in-c-elegans/13463/9 "2024-04-21T14:21:50Z")
Another drug (zidovudine) which affects mitochondria and inhibits translation also increases lifespan in C elegans. > **[Anti-retroviral treatment with zidovudine alters pyrimidine metabolism,...](https://pubmed.ncbi.nlm.nih.gov/36640360/)** > > The human population is aging, and the need for interventions to slow progression of age-related diseases (geroprotective interventions) is growing. Repurposing compounds already used clinically, usually at modified doses, allows rapid implementation... Personally I think the RTI activity of zidovudine is more interesting when it comes to mammalian aging, as this could prevent retrotransposition events. These are probably less of a concern in C elegans, where in contrast to mammals, a very small portion of the genome is occupied by retrotranspons. > **[Transposons in C. elegans](https://www.ncbi.nlm.nih.gov/books/NBK19727/#_A921_)** > > Transposons are discrete segments of DNA capable of moving through the genome of their host via an RNA intermediate in the case of class I retrotransposon or via a "cut-and-paste" mechanism for class II DNA transposons. Since transposons take... ---
**Author:** ![desertshores](https://www.rapamycin.news/user_avatar/www.rapamycin.news/desertshores/32/17120_2.png) [@desertshores](https://www.rapamycin.news/u/desertshores)\ **Post date:** [April 21, 2024, 5:02pm UTC](https://www.rapamycin.news/t/antibiotics-that-target-mitochondria-extend-lifespan-in-c-elegans/13463/10 "2024-04-21T17:02:36Z")
> [@My DAV\* Therapy Experiment begins! \*Doxycycline, Azithromycin and Vitamin C](https://www.rapamycin.news/t/my-dav-therapy-experiment-begins-doxycycline-azithromycin-and-vitamin-c/9619/124): > > Patients who took amoxicillin had no increase in the risk of death during this period. Vitamin C, which I have previously commented on seems to add nothing to the protocol. ---
**Author:** ![Deborah\_Hall](https://www.rapamycin.news/user_avatar/www.rapamycin.news/deborah_hall/32/23064_2.png) [@Deborah\_Hall](https://www.rapamycin.news/u/Deborah_Hall)\ **Post date:** [April 21, 2024, 5:53pm UTC](https://www.rapamycin.news/t/antibiotics-that-target-mitochondria-extend-lifespan-in-c-elegans/13463/11 "2024-04-21T17:53:54Z")
Antibiotic, along with a statin, and metformin and an “anti-helminth” are the basic four elements of the Jane McClelland “Starve Cancer” protocol which grew partially from Lisanti’s work and then became the basis for the “Care Oncology Protocol”. There is a beautiful logic for each of these four which becomes really clear if you read the Jason Fung book The Cancer Code. Highly recommend McClelland and Fung even if you are not actively pursuing an anti-cancer strategy. ---
**Author:** ![Joseph](https://www.rapamycin.news/letter_avatar_proxy/v4/letter/j/dc4da7/32.png) [@Joseph](https://www.rapamycin.news/u/Joseph)\ **Post date:** [April 21, 2024, 8:00pm UTC](https://www.rapamycin.news/t/antibiotics-that-target-mitochondria-extend-lifespan-in-c-elegans/13463/12 "2024-04-21T20:00:44Z")
> [@desertshores](#): > > _“Vitamin C, which I have previously commented on seems to add nothing to the protocol.”_ The Vitiman C works{in the DAV Therapy]as a pro oxidant to kill off the senescent cells that have been rounded up by the doxycycline (works on small mitochondrial and the azithromycin(works on the large mitochondrial) As stated{by Professor LIsanti] in the paper and in the interview the azithromycin killed off 97% of senescent cells. Better than dasatinib and, quercetin, He used CD26 as the senescent marker. Interview during COVID [![](https://img.youtube.com/vi/2DvJwthiMPk/maxresdefault.jpg "Interview with Professor Michael Lisanti, M.D., PhD") ](https://www.youtube.com/watch?v=2DvJwthiMPk) Doing DAV Therapy is safer that using dasatinib and cost very little lless than $35.00 for the 5 weeks And I will post it again, if you understand the [paper](https://www.aging-us.com/article/101905)… In my view; It is a no brainer!